Autotoxicity in cucumber (Cucumis sativus L.) seedlings is alleviated by silicon through an increase in the activity of antioxidant enzymes and by mitigating lipid peroxidation

被引:47
作者
Bu, Ruifang [1 ]
Xie, Jianming [1 ]
Yu, Jihua [1 ]
Liao, Weibiao [1 ]
Xiao, Xuemei [1 ]
Lv, Jian [1 ]
Wang, Cuili [1 ]
Ye, Jie [1 ]
Calderon-Urrea, Alejandro [2 ]
机构
[1] Gansu Agr Univ, Coll Hort, Lanzhou 730070, Peoples R China
[2] Calif State Univ Fresno, Dept Biol, Fresno, CA 93711 USA
基金
中国国家自然科学基金;
关键词
Antioxidant enzymes; Cucumber seed germination; Lipid peroxidation; Non-enzymatic antioxidant; 3-phenylpropionic acid (PA); Reactive oxygen species; SUPEROXIDE-DISMUTASE; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; MEDIATED ALLEVIATION; SCAVENGING ENZYME; ASCORBIC-ACID; H+-ATPASE; GLUTATHIONE; LEAVES; OXYGEN;
D O I
10.1007/s12374-016-0526-1
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Autotoxicity in plants limits their growth and that of nearby plants of the same species, which has obvious implications in crop yield and quality. Silicon (Si) has been shown to increase plant tolerance to autotoxic stress. However, the physiological mechanisms underlying the effects of Si in alleviating autotoxicity during germination in cucumber (Cucumis sativus L.) are unknown. Cinnamic acid derivatives, such as 3-phenylpropionic acid (PA), are a class of autotoxins present in cucumber root exudates. Our objective was to investigate Si-induced autotoxic stress tolerance in cucumber seedlings by focusing on the effects of Si on the induction of antioxidant defense pathways. We found that PA treatment significantly reduced seed germination, radicle length, lateral root number, fresh weight, AsA and GSH contents, and the activities of SOD, CAT, and APX in cucumber seedlings, while it increased membrane permeability and levels of MDA, proline, O (2) (-) , and H2O2. Application of Si enhanced growth of PA-treated plants and significantly increased germination rate, radicle length, lateral root number, fresh weight, AsA and GSH levels, and SOD, CAT, POD, and APX activities. These results suggest that exogenous Si alleviates autotoxicity caused by PA during seed germination by increasing antioxidant enzyme activities and mitigating lipid peroxidation.
引用
收藏
页码:247 / 259
页数:13
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