Strategies of genome editing in mycobacteria: Achievements and challenges

被引：12
作者：
Choudhary, Eira [1 ,2 ]
Lunge, Ajitesh [1 ,3 ]
Agarwal, Nisheeth [1 ]
机构：
[1] NCR Biotech Sci Cluster, Translat Hlth Sci & Technol Inst, 3rd Milestone, Faridabad 121001, Haryana, India
[2] Symbiosis Int Univ, Symbiosis Sch Biomed Sci, Pune 412115, Maharashtra, India
[3] Jawaharlal Nehru Univ, New Mehrauli Rd, Delhi 110067, India
来源：
TUBERCULOSIS | 2016年 / 98卷
关键词：
Mycobacterium tuberculosis; Homologous recombination; Genome editing; CRISPRi; MULTIDRUG-RESISTANCE GENE; CONDITIONAL EXPRESSION; CELL-DIVISION; ANTISENSE OLIGONUCLEOTIDES; INDUCIBLE ACETAMIDASE; CRISPR INTERFERENCE; ADAPTIVE IMMUNITY; BOVIS BCG; TUBERCULOSIS; SMEGMATIS;
D O I：
10.1016/j.tube.2016.03.005
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Tremendous amount of physiological and functional complexities acquired through decades of evolutionary pressure makes Mycobacterium tuberculosis (Mtb) one of the most dreadful microorganisms infecting humans from centuries. Astonishing advances in genomics and genome editing tools substantially grew our knowledge about Mtb as an organism but dramatically failed to completely understand it as a pathogen. Though conventional tools based on homologous recombination, antisense, controlled proteolysis, etc. have made important contributions in advancing our understanding of the pathophysiology of Mtb, yet these approaches have not accentuated our exploration of mycobacterium on account of certain technical limitations. In this review article we have compiled various approaches implemented in genome editing of mycobacteria along with the latest adaptation of clustered regularly interspaced short palindromic repeat (CRISPR)-interference (CRISPRi), emphasizing the achievements and challenges associated with these techniques. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：132 / 138
页数：7
相关论文
共 64 条
[1]  
Agarwal N., UNPUB
[2]   THE URAA LOCUS AND HOMOLOGOUS RECOMBINATION IN MYCOBACTERIUM-BOVIS BCG [J].
ALDOVINI, A ;
HUSSON, RN ;
YOUNG, RA .
JOURNAL OF BACTERIOLOGY, 1993, 175 (22) :7282-7289
[3]   Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates [J].
Balasubramanian, V ;
Pavelka, MS ;
Bardarov, SS ;
Martin, J ;
Weisbrod, TR ;
McAdam, RA ;
Bloom, BR ;
Jacobs, WR .
JOURNAL OF BACTERIOLOGY, 1996, 178 (01) :273-279
[4]   Specialized transduction:: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M-bovis BCG and M-smegmatis [J].
Bardarov, S ;
Bardarov, S ;
Pavelka, MS ;
Sambandamurthy, V ;
Larsen, M ;
Tufariello, J ;
Chan, J ;
Hatfull, G ;
Jacobs, WR .
MICROBIOLOGY-SGM, 2002, 148 :3007-3017
[5]   Gene regulation by tetracyclines - Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes [J].
Berens, C ;
Hillen, W .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (15) :3109-3121
[6]   MOLECULAR CHARACTERIZATION AND TRANSCRIPTIONAL ANALYSIS OF A MULTIDRUG-RESISTANCE GENE CLONED FROM THE PRISTINAMYCIN-PRODUCING ORGANISM, STREPTOMYCES-PRISTINAESPIRALIS [J].
BLANC, V ;
SALAHBEY, K ;
FOLCHER, M ;
THOMPSON, CJ .
MOLECULAR MICROBIOLOGY, 1995, 17 (05) :989-999
[7]   Global analysis of proteins synthesized by Mycobacterium smegmatis provides direct evidence for physiological heterogeneity in stationary-phase cultures [J].
Blokpoel, MCJ ;
Smeulders, MJ ;
Hubbard, JAM ;
Keer, J ;
Williams, HD .
JOURNAL OF BACTERIOLOGY, 2005, 187 (19) :6691-6700
[8]   Development of a repressible mycobacterial promoter system based on two transcriptional repressors [J].
Boldrin, Francesca ;
Casonato, Stefano ;
Dainese, Elisa ;
Sala, Claudia ;
Dhar, Neeraj ;
Palu, Giorgio ;
Riccardi, Giovanna ;
Cole, Stewart T. ;
Manganelli, Riccardo .
NUCLEIC ACIDS RESEARCH, 2010, 38 (12) :e134
[9]   Small CRISPR RNAs guide antiviral defense in prokaryotes [J].
Brouns, Stan J. J. ;
Jore, Matthijs M. ;
Lundgren, Magnus ;
Westra, Edze R. ;
Slijkhuis, Rik J. H. ;
Snijders, Ambrosius P. L. ;
Dickman, Mark J. ;
Makarova, Kira S. ;
Koonin, Eugene V. ;
van der Oost, John .
SCIENCE, 2008, 321 (5891) :960-964
[10]   Instability of the acetamide-inducible expression vector pJAM2 in Mycobacterium tuberculosis [J].
Brown, AC ;
Parish, T .
PLASMID, 2006, 55 (01) :81-86
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