In vivo examination of membrane protein localization and degradation with green fluorescent protein

被引：105

作者：

Hampton, RY

Koning, A

Wright, R

Rine, J

机构：

[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, DIV GENET, BERKELEY, CA 94720 USA

[2] UNIV WASHINGTON, DEPT ZOOL, SEATTLE, WA 98195 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 02期

关键词：

D O I：

10.1073/pnas.93.2.828

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP, Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.

引用

页码：828 / 833

页数：6

共 12 条

[1] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].