A novel visible detection strategy for lysozyme based on gold nanoparticles and conjugated polymer brush

被引：19
作者：
Gu, Peng [1 ,2 ]
Liu, Xingfen [1 ,2 ]
Tian, Yuanyuan [1 ,2 ]
Zhang, Lei [1 ,2 ]
Huang, Yanqin [1 ,2 ]
Su, Shao [1 ,2 ]
Feng, Xiaomiao [1 ,2 ]
Fan, Quli [1 ,2 ]
Huang, Wei [3 ,4 ]
机构：
[1] Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat, Key Lab Organ Elect & Informat Displays, Nanjing 210023, Jiangsu, Peoples R China
[2] Nanjing Univ Posts & Telecommun, Jiangsu Natl Synerget Innovat Ctr Adv Mat, Inst Adv Mat, Nanjing 210023, Jiangsu, Peoples R China
[3] Nanjing Tech Univ Nanjing Tech, Jiangsu Natl Synerget Innovat Ctr Adv Mat, Key Lab Flexible Elect, Nanjing 211816, Jiangsu, Peoples R China
[4] Nanjing Tech Univ Nanjing Tech, Jiangsu Natl Synerget Innovat Ctr Adv Mat, Inst Adv Mat, Nanjing 211816, Jiangsu, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 246卷
关键词：
Biosensor; Conjugated polymer; Gold nanoparticle; Lysozyme; HIGH CHARGE-DENSITY; IN-VITRO SELECTION; COLORIMETRIC DETECTION; ENERGY-TRANSFER; QUANTUM DOTS; POLYELECTROLYTE BRUSH; DNA; APTAMER; EFFICIENT; DISEASE;
D O I：
10.1016/j.snb.2017.01.043
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We developed a new strategy by combining gold nanoparticles (AuNPs) with a fluorescent conjugated polymer brush (ThNI) for colorimetric and fluorometric detection of human lysozyme. This strategy was successfully applied to assay lysozyme in buffer and human saliva. In comparison with previous reported methods, our approach has the advantages of simpleness, sensitivity, label-free property, and low cost. (C) 2017 Published by Elsevier B.V.
引用
收藏
页码：78 / 84
页数：7
相关论文
共 54 条
[1]  
[Anonymous], 2012, THESIS
[2]   Colorimetric detection of lysozyme based on electrostatic interaction with human serum albumin-modified gold nanoparticles [J].
Chen, Yi-Ming ;
Yu, Cheng-Ju ;
Cheng, Tian-Lu ;
Tseng, Wei-Lung .
LANGMUIR, 2008, 24 (07) :3654-3660
[3]   INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].
ELLINGTON, AD ;
SZOSTAK, JW .
NATURE, 1990, 346 (6287) :818-822
[4]   Beyond superquenching: Hyper-efficient energy transfer from conjugated polymers to gold nanoparticles [J].
Fan, CH ;
Wang, S ;
Hong, JW ;
Bazan, GC ;
Plaxco, KW ;
Heeger, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6297-6301
[5]   Conjugated polymer nanoparticles: preparation, properties, functionalization and biological applications [J].
Feng, Liheng ;
Zhu, Chunlei ;
Yuan, Huanxiang ;
Liu, Libing ;
Lv, Fengting ;
Wang, Shu .
CHEMICAL SOCIETY REVIEWS, 2013, 42 (16) :6620-6633
[6]   Chemiluminescent and Chemiluminescence Resonance Energy Transfer (CRET) Detection of DNA, Metal Ions, and Aptamer-Substrate Complexes Using Hemin/G-Quadruplexes and CdSe/ZnS Quantum Dots [J].
Freeman, Ronit ;
Liu, Xiaoqing ;
Willner, Itamar .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (30) :11597-11604
[7]   Development of a novel fluorescent microbead-based immunoassay and comparison with three enzyme-linked immunoassays for detection of anti-Erysipelothrix spp. IgG antibodies in pigs with known and unknown exposure [J].
Gimenez-Lirola, L. G. ;
Xiao, C. T. ;
Halbur, P. G. ;
Opriessnig, T. .
JOURNAL OF MICROBIOLOGICAL METHODS, 2012, 91 (01) :73-79
[8]   Colorimetric detection of controlled assembly and disassembly of aptamers on unmodified gold nanoparticles [J].
Gopinath, Subash C. B. ;
Lakshmipriya, Thangavel ;
Awazu, Koichi .
BIOSENSORS & BIOELECTRONICS, 2014, 51 :115-123
[9]   Aptamer beacons for the direct detection of proteins [J].
Hamaguchi, N ;
Ellington, A ;
Stanton, M .
ANALYTICAL BIOCHEMISTRY, 2001, 294 (02) :126-131
[10]   Water-soluble conjugated polyelectrolyte brush encapsulated rare-earth ion doped nanoparticles with dual-upconversion properties for multicolor cell imaging [J].
Hu, Wenbo ;
Lu, Xiaomei ;
Jiang, Rongcui ;
Fan, Quli ;
Zhao, Hui ;
Deng, Weixing ;
Zhang, Lei ;
Huang, Ling ;
Huang, Wei .
CHEMICAL COMMUNICATIONS, 2013, 49 (79) :9012-9014
123456