A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum

被引：52

作者：

Bei, Amy K. ^{[1
]}

DeSimone, Tiffany M. ^{[1
]}

Badiane, Aida S. ^{[2
,3
]}

Ahouidi, Ambroise D. ^{[2
,3
]}

Dieye, Tandakha ^{[2
,3
]}

Ndiaye, Daouda ^{[2
,3
]}

Sarr, Ousmane ^{[2
,3
]}

Ndir, Omar ^{[2
,3
]}

Mboup, Souleymane ^{[2
,3
]}

Duraisingh, Manoj T. ^{[1
]}

机构：

[1] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA

[2] Cheikh Anta Diop Univ, Fac Med & Pharm, Parasitol Lab, Dakar, Senegal

[3] Cheikh Anta Diop Univ, Fac Med & Pharm, Dantec Hosp, Lab Bacteriol & Virol, Dakar, Senegal

来源：

AMERICAN JOURNAL OF HEMATOLOGY | 2010年 / 85卷 / 04期

关键词：

SYBR-GREEN-I; MALARIA; ERYTHROCYTES; PARASITEMIA; VALIDATION; SEVERITY;

D O I：

10.1002/ajh.21642

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion. Am. J. Hematol. 85:234-237, 2010. (C) 2010 Wiley-Liss, Inc.

引用

页码：234 / 237

页数：4

共 14 条

[1]

AMINOFF D, 1988, BLOOD CELLS, V14, P229

[2] Erythrocyte invasion phenotypes of Plasmodium falciparum in the Gambia [J].