The polymorphisms of stearoyl-CoA desaturase (SCD1) and sterol regulatory element binding protein-1 (SREBP-1) genes and their association with the fatty acid profile of muscle and subcutaneous fat in Fleckvieh bulls

被引：50

作者：

Barton, L. ^{[1
]}

Kott, T. ^{[1
]}

Bures, D. ^{[1
]}

Rehak, D. ^{[1
]}

Zahradkova, R. ^{[1
]}

Kottova, B. ^{[1
]}

机构：

[1] Inst Anim Sci, CZ-10401 Prague 10, Czech Republic

来源：

MEAT SCIENCE | 2010年 / 85卷 / 01期

关键词：

Cattle; Adipose tissue; Fatty acid composition; Polymorphism; Stearoyl-CoA desaturase (SCD1); Sterol regulatory element binding protein-1 (SREBP-1); CONJUGATED LINOLEIC-ACID; ADIPOSE-TISSUE; TRANSCRIPTION FACTORS; ENZYME-ACTIVITIES; MEAT; EXPRESSION; IDENTIFICATION; HOLSTEIN; HEALTH; LIVER;

D O I：

10.1016/j.meatsci.2009.11.016

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The previously reported genetic polymorphisms of the stearoyl-CoA desaturase (SCD1) and sterol regulatory element binding protein-1 (SREBP-1) genes were investigated in Fleckvieh bulls using the PCR-RFLP and AS-PCR methods, respectively. The genomic DNA was obtained from a total of 370 bulls. The frequencies of alleles A and V of the single nucleotide polymorphism in exon 5 of the SCD1 gene (SNP 878C > T) were 0.555 and 0.445, respectively. In the 84-bp Ins/Del polymorphism in intron 5 of the SREBP-1 gene, the frequency of the L allele (insertion) was markedly higher (0.920) than that of the S allele (deletion; 0.080). Fatty acid profile was determined in a total of 367 samples of muscle fat (MSF) and 150 samples of subcutaneous fat (SCF). The AA genotype of SCD1 polymorphism showed a lower content of C18:0 (P < 0.01) and higher contents of C14:1 cis-9 (P < 0.001) and C18:1 cis-9 (P < 0.05) in MSF compared to the VV genotype. As a result, the bulls with genotypes AA or AV had lower SEA (P < 0.01), higher MUFA (P < 0.05) and higher MUFA/SFA (P < 0.01)than VV animals. The results obtained for SCF were similar. The SREBP-1 polymorphism was associated with a higher content of C14:1 cis-9 (P < 0.01) in the LS compared to LL genotype in SCF. The results of this study demonstrated the existence of the polymorphisms in the SCD1 and SREBP-1 genes in the population of Fleckvieh cattle and their associations with the concentrations of several MSF and SCF fatty acids. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：15 / 20

页数：6

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