Delivery of Macromolecules into Live Cells by Simple Co-incubation with a Peptide

被引：30

作者：

Lee, Ya-Jung ^{[1
]}

Erazo-Oliveras, Alfredo ^{[1
]}

Pellois, Jean-Philippe ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 03期

关键词：

cell-penetrating peptides; delivery; imaging agents; membranes; proteins; INFLUENZA-VIRUS HEMAGGLUTININ; BIOLOGICALLY-ACTIVE PROTEINS; ARGININE-RICH PEPTIDES; MAMMALIAN-CELLS; MEMBRANE-FUSION; PENETRATING PEPTIDES; CELLULAR UPTAKE; GENE DELIVERY; LIVING CELLS; MACROPINOCYTOSIS;

D O I：

10.1002/cbic.200900527

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) Special delivery: Macromolecules are delivered to live cells by simple co-incubation with the peptide E5-TAT. The peptide induces endocytic uptake of macromolecules present in the media surrounding cells. The peptide then responds to the acidification of endocytic organelles to promote the cytosolic release of the macromolecules with which it is sequestered. A critical advantage of this approach is that the macromolecules do not need to be conjugated to the delivery peptide. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：325 / 330

页数：6

共 41 条

[1] Delivery of proteins and nucleic acids using a non-covalent peptide-based strategy [J].

Deshayes, Sebastien ;

Morris, May ;

Heitz, Frederic ;

Divita, Gilles .

ADVANCED DRUG DELIVERY REVIEWS, 2008, 60 (4-5) :537-547

[2] Anti-cancer activity of targeted pro-apoptotic peptides [J].

Ellerby, HM ;

Arap, W ;

Ellerby, LM ;

Kain, R ;

Andrusiak, R ;

Del Rio, G ;

Krajewski, S ;

Lombardo, CR ;

Rao, R ;

Ruoslahti, E ;

Bredesen, DE ;

Pasqualini, R .

NATURE MEDICINE, 1999, 5 (09) :1032-1038

[3] Delivery of oligonucleotides into mammalian cells by anionic peptides: comparison between monomeric and dimeric peptides [J].

Freulon, I ;

Roche, AC ;

Monsigny, M ;

Mayer, R .

BIOCHEMICAL JOURNAL, 2001, 354 :671-679

[4] Systematic solid-phase synthesis of linear pseudooligolysines containing multiple adjacent CH2NH amide bond surrogates:: potential agents for gene delivery [J].

Fridkin, G ;

Gilon, T ;

Loyter, A ;

Gilon, C .

JOURNAL OF PEPTIDE RESEARCH, 2001, 58 (01) :36-44

[5] Transport between the cell nucleus and the cytoplasm [J].

Görlich, D ;

Kutay, U .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1999, 15 :607-660

[6] Structural characterizations of fusion peptide analogs of influenza virus hemagglutinin - Implication of the necessity of a helix-hinge-helix motif in fusion activity [J].

Hsu, CH ;

Wu, SH ;

Chang, DK ;

Chen, CP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (25) :22725-22733

[7] De novo antimicrobial peptides with low mammalian cell toxicity [J].

Javadpour, MM ;

Juban, MM ;

Lo, WCJ ;

Bishop, SM ;

Alberty, JB ;

Cowell, SM ;

Becker, CL ;

McLaughlin, ML .

JOURNAL OF MEDICINAL CHEMISTRY, 1996, 39 (16) :3107-3113

[8] Defining Macropinocytosis [J].

Kerr, Markus C. ;

Teasdale, Rohan D. .

TRAFFIC, 2009, 10 (04) :364-371

[9] Histidine-rich amphipathic peptide antibiotics promote efficient delivery of DNA into mammalian cells [J].

Kichler, A ;

Leborgne, C ;

März, J ;

Danos, O ;

Bechinger, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) :1564-1568

[10] Delivery and visualization of proteins conjugated to quantum dots in cardiac myocytes [J].

Koshman, Yevgeniya E. ;

Waters, Stephen B. ;

Walker, Lori A. ;

Los, Tamara ;

de Tombe, Pieter ;

Goldspink, Paul H. ;

Russell, Brenda .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2008, 45 (06) :853-856

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