Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH

被引：28

作者：

Al-Ali, Hassan ^{[1
]}

Rieger, Megan E. ^{[1
,2
]}

Seldeen, Kenneth L. ^{[1
,2
]}

Harris, Thomas K. ^{[1
]}

Farooq, Amjad ^{[1
,2
]}

Briegel, Karoline J. ^{[1
,2
]}

机构：

[1] Univ Miami, Miller Sch Med, Dept Biochem & Mol Biol, Miami, FL 33136 USA

[2] Univ Miami, Miller Sch Med, Braman Family Breast Canc Inst, Sylvester Comprehens Canc Ctr, Miami, FL 33136 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 01期

关键词：

Vertebrates; LBH; Transcriptional regulation; Embryonic development; Intrinsically disordered proteins; INTRINSIC DISORDER; PROTEIN; SPECTRA;

D O I：

10.1016/j.bbrc.2009.12.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Limb-bud and heart (LBH) is a key transcriptional regulator in vertebrates with pivotal roles in embryonic development and human disease. Herein, using a diverse array of biophysical techniques, we report the first structural characterization of LBH pertinent to its biological function. Our data reveal that LBH is structurally disordered with no discernable secondary or tertiary Structure and exudes rod-like properties in Solution. Consistent with these observations, we also demonstrate that LBH is conformationally flexible and thus may be capable of adapting distinct conformations under specific physiological contexts. We propose that LBH is a member of the intrinsically disordered protein (IDP) family, and that conformational plasticity may play a significant role in modulating LBH-dependent transcriptional processes. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1104 / 1109

页数：6

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