Evaluation of the safety of time-lapse observations for human embryos

被引：82

作者：

Nakahara, Tatsuo ^{[1
]}

Iwase, Akira ^{[1
]}

Goto, Maki ^{[1
]}

Harata, Toko ^{[1
,2
]}

Suzuki, Miyabi ^{[2
]}

Ienaga, Miki ^{[1
]}

Kobayashi, Harumi ^{[1
]}

Takikawa, Sachiko ^{[1
]}

Manabe, Shuichi ^{[1
]}

Kikkawa, Fumitaka ^{[1
]}

Ando, Hisao ^{[1
,3
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Obstet & Gynecol, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Toyota Mem Hosp, Ctr Infertil, Toyota, Japan

[3] Toyohashi Municipal Hosp, Ctr Reprod Med, Toyohashi, Aichi, Japan

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2010年 / 27卷 / 2-3期

关键词：

Built-in microscope incubator; Embryo grade; ICSI; Time-lapse observation; INTRACYTOPLASMIC SPERM INJECTION; IN-VITRO FERTILIZATION; EARLY CLEAVAGE; PREGNANCY RATES; 2-CELL STAGE; MORPHOLOGY; QUALITY; INDICATOR; IMPLANTATION; VIABILITY;

D O I：

10.1007/s10815-010-9385-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To assess the effects of light from an integrated optical microscope and evaluate the safety of time-lapse observations using a built-in microscope incubator. We prospectively compared the fertilization rate and embryonic morphology after intracytoplasmic sperm injection between embryos cultured with time-lapse observations every 15 min in an incubator with an integrated optical microscope and embryos with intermittent observations (once a day) in conventional incubators. No significant differences were observed in the fertilization rate (57.5% vs. 57.5%) or the rate of excellent-good cleavage embryos (36.0% vs. 36.0%). These results suggest that time-lapse observations using an incubator with an integrated optical microscope may therefore be safely utilized in clinical practice.

引用

页码：93 / 96

页数：4

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