Characterization of CKβ8 and CKβ8-1:: Two alternatively spliced forms of human β-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1

被引:76
作者
Youn, BS
Zhang, SM
Broxmeyer, HE
Cooper, S
Antol, K
Fraser, M
Kwon, BS
机构
[1] Indiana Univ, Sch Med, Dept Immunol & Microbiol, Indianapolis, IN 46202 USA
[2] Indiana Univ, Sch Med, Walther Oncol Ctr, Indianapolis, IN 46202 USA
[3] St Marys Coll, Dept Chem Phys, Notre Dame, IN 46556 USA
[4] Univ Notre Dame, Dept Biol Sci, S Bend, IN USA
关键词
D O I
10.1182/blood.V91.9.3118.3118_3118_3126
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Two new members of human beta-chemokine cDNA were isolated based on structural and functional similarities to human leukotactin-1. One of these clones was identical to the previously isolated human beta-chemokine, CK beta 8, whereas the other is a splicing variant of CK beta 8, therefore named CK beta 8-1. CK beta 8 was short in 51 nucleotides (17 amino acids) compared with CK beta 8-1. The mature proteins of CK beta 8-1 and CK beta 8 consisted of 116 and 99 amino acids with calculated molecular weights of 12,500 and 10,950, respectively. Both CK beta-1 and CK beta 8 were potent agonists at CCR1. These chemokines chemoattracted neutrophils, monocytes, and lymphocytes, They also significantly suppressed colony formation by human bone marrow, granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. To our knowledge, this is the first example that an alternative splicing produces two active beta-chemokines from a single gene. (C) 1998 by The American Society of Hematology.
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收藏
页码:3118 / 3126
页数:9
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