Identification of Chemical Markers for the Discrimination of Radix Angelica sinensis Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics

被引：28

作者：

Zhang, Kaixue ^{[1
]}

Yan, Menglin ^{[2
]}

Han, Shu ^{[3
]}

Cong, Longfei ^{[2
]}

Wang, Liyao ^{[1
]}

Zhang, Liu ^{[1
]}

Sun, Lili ^{[1
]}

Bai, Haiying ^{[1
]}

Wei, Guanhua ^{[1
]}

Du, Hong ^{[3
]}

Jiang, Min ^{[2
]}

Bai, Gang ^{[2
]}

Yang, Zhigang ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China

[2] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300350, Peoples R China

[3] Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 100029, Peoples R China

来源：

MOLECULES | 2019年 / 24卷 / 19期

关键词：

Angelica sinensis; geoherb region; chemical markers; content; UHPLC-QTOF-MS; MS; metabolomics; anti-inflammation; calcium antagonists;

D O I：

10.3390/molecules24193536

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs.

引用

页数：13

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