Heterobifunctional poly(ethylene glycol)-tethered bone morphogenetic protein-2-stimulated bone marrow mesenchymal stromal cell differentiation and osteogenesis

被引:62
作者
Liu, Hsia-Wei
Chen, Chih-Hwa
Tsai, Ching-Lin
Lin, I-Hsuan
Hsiue, Ging-Ho
机构
[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu 30013, Taiwan
[2] Chang Gung Univ, Dept Orthoped Surg, Coll Med, Chang Gung Mem Hosp Keelung, Chilung, Taiwan
[3] Natl Taiwan Univ Hosp, Coll Med, Dept Orthoped Surg, Taipei, Taiwan
来源
TISSUE ENGINEERING | 2007年 / 13卷 / 05期
关键词
D O I
10.1089/ten.2006.0209
中图分类号
Q813 [细胞工程];
学科分类号
摘要
We describe a biomimetic mode of insoluble signaling stimulation to provide target delivery of bone morphogenetic protein-2 (BMP-2), with the aim of prolonging the retention of BMP-2 use in bone tissue engineering and to enable its localized release in response to cellular activity. In our novel localization process, we used heterobifunctional acrylate-N-hydroxysuccinimide poly(ethylene glycol) (PEG) as a spacer to tether BMP-2 onto a poly(lactide-co-glycolide) scaffold. Use of PEG-tethered BMP-2 was feasible because BMP-2 retained its activity after covalent conjugation. The PEG-tethered BMP-2 conjugate sustained stimulation and retained its mitogenic activity, notably affecting pluripotent stem cell proliferation and differentiation. We seeded the scaffolds with bone marrow-derived mesenchymal stromal cells as progenitor cells to evaluate their morphology and phenotypic expression. We also created bilateral, fullthickness cranial defects in rabbits to investigate the osteogenic effect of cultured mesenchymal stromal cells on bone regeneration in vivo. Histomorphometry and histology demonstrated that the PEG-tethered BMP-2 conjugate enhanced de novo bone formation after surgery. Our work revealed the potential for biomimetic surface engineering by entrapping signaling growth factor to stimulate osteogenesis. Our technique may provide a new platform for bone-engineered stem cell therapies.
引用
收藏
页码:1113 / 1124
页数:12
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