Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR

被引：34
作者：
Deurenberg, RH
Vink, C
Driessen, C
Bes, M
London, N
Etienne, J
Stobberingh, EE
机构：
[1] Univ Hosp Maastricht, Dept Med Microbiol, NL-6202 AZ Maastricht, Netherlands
[2] Fac Med Laennec, INSERM E0230, IFR 62, Lyon, France
来源：
FEMS MICROBIOLOGY LETTERS | 2004年 / 240卷 / 02期
关键词：
Panton-Valentine leukocidin; S; aureus; real-time PCR;
D O I：
10.1016/j.femsle.2004.09.031
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：225 / 228
页数：4
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