Excitation of primary afferent neurons by near-infrared light in vitro

被引:23
作者
Katz, Elizabeth J. [1 ]
Ilev, Ilko K. [1 ]
Krauthamer, Victor [1 ]
Kim, Do Hyun [1 ]
Weinreich, Daniel [1 ,2 ]
机构
[1] US FDA, Ctr Devices & Radiol Hlth, Silver Spring, MD 20993 USA
[2] Sch Med, Dept Pharmacol, Baltimore, MD USA
关键词
dorsal root ganglion; laser; near-infrared light; nodose ganglion; optical stimulation; ROOT GANGLION NEURONS; OPTICAL STIMULATION; RECEPTOR; EXCITABILITY; EXPRESSION; CAPSAICIN; PROTON; TISSUE; CELLS;
D O I
10.1097/WNR.0b013e32833add3a
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Near-infrared light therapy is an emerging neurostimulation technology, but its cellular mechanism of action remains unresolved. Using standard intracellular recording techniques, we observed that 5-10ms pulses of 1889 nm light depolarized the membrane potential for hundreds of milliseconds in more than 85% of dorsal root ganglion and nodose ganglion neurons tested. The laser-evoked depolarizations (LEDs) exhibited complex, multiphasic kinetics comprising fast and slow components. There was no discernable difference in the LEDs in intact ganglion neurons and in acutely isolated neurons. Thus, the LED sensor seems to reside within the neuronal membrane. The near-uniform distribution of responsive neurons increased membrane conductance, and the negative reversal potential value (-41 +/- 2.9 mV) suggests that LED is unrelated to the activation of heat-sensitive transient receptor potential cation channel subfamily V member 1 channels. The long duration of LEDs favors an involvement of second messengers. NeuroReport 21: 662-666 (C) 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.
引用
收藏
页码:662 / 666
页数:5
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