A Two-Dimensional Metallacycle Cross-Linked Switchable Polymer for Fast and Highly Efficient Phosphorylated Peptide Enrichment

被引：35

作者：

Chen, Li-Jun ^{[1
]}

Humphrey, Sean J. ^{[2
]}

Zhu, Jun-Long ^{[3
]}

Zhu, Fan-Fan ^{[3
]}

Wang, Xu-Qing ^{[3
]}

Wang, Xiang ^{[4
,5
]}

Wen, Jin ^{[4
,5
,6
]}

Yang, Hai-Bo ^{[3
]}

Gale, Philip A. ^{[1
,7
]}

机构：

[1] Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia

[2] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia

[3] East China Normal Univ, Chang Kung Chuang Inst, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai 200062, Peoples R China

[4] Donghua Univ, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China

[5] Donghua Univ, Coll Mat Sci & Engn, Shanghai 201620, Peoples R China

[6] Univ Vienna, Fac Vienna, Inst Theoret Chem, A-1090 Vienna, Austria

[7] Univ Sydney, Nano Inst Sydney Nano, Sydney, NSW 2006, Australia

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2021年 / 143卷 / 22期

基金：

奥地利科学基金会; 澳大利亚研究理事会;

关键词：

ANION RECOGNITION; RATIONAL DESIGN; COORDINATION; COMPLEXATION;

D O I：

10.1021/jacs.0c12904

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The selective and efficient capture of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis. Here we report a new switchable two-dimensional (2D) supramolecular polymer that serves as an ideal platform for the enrichment of phosphopeptides. A well-defined, positively charged metallacycle incorporated into the polymer endows the resultant polymer with a high affinity for phosphopeptides. Importantly, the stimuli-responsive nature of the polymer facilitates switchable binding affinity of phosphopeptides, thus resulting in an excellent performance in phosphopeptide enrichment and separation from model proteins. The polymer has a high enrichment capacity (165 mg/g) and detection sensitivity (2 fmol), high enrichment recovery (88%), excellent specificity, and rapid enrichment and separation properties. Additionally, we have demonstrated the capture of phosphopeptides from the tryptic digest of real biosamples, thus illustrating the potential of this polymeric material in phosphoproteomic studies.

引用

页码：8295 / 8304

页数：10

共 57 条

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