Studies on mRNA expression of tissue-type plasminogen activator in bruises for wound age estimation

被引:41
作者
Takamiya, M [1 ]
Saigusa, K [1 ]
Kumagai, R [1 ]
Nakayashiki, N [1 ]
Aoki, Y [1 ]
机构
[1] Iwate Med Univ, Sch Med, Dept Legal Med, Morioka, Iwate 0208505, Japan
关键词
tissue-type plasminogen activator; bruise; wound aging; quantitative pcr; in situ hybridization;
D O I
10.1007/s00414-004-0453-4
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
We investigated mRNA expression of tissue-type plasminogen activator (tPA) and inflammatory cell dynamics for wound age estimation of bruises in mice. Neutrophils were detected from 1 h post-injury. Up to 8 h, they accumulated in subcutaneous tissue and the lower part of the dermis, and thereafter they extended to all the layers. Macrophages became detectable 3 h post-injury, and moderate infiltration of lymphocytes was seen from 144 h. In addition, epidermal thickening was also seen from 72 h. tPA mRNA expression peaked at 1 h, and increased slightly at 72 h post-injury. tPA mRNA was detected in epidermal cells, fibroblasts, and endothelial cells before and after injury, from 3 h in neutrophils and from 72 h in macrophages, respectively. This study presents the time-dependent expression of tPA mRNA in bruises in relation to temporal histologic characteristics during wound healing, which was considered to be useful for wound age estimation. Furthermore, it is suggested that tPA plays an important role in the first step of tissue remodeling.
引用
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页码:16 / 21
页数:6
相关论文
共 20 条
[1]  
ANGGARD E, 1972, HOSP TRIB, V6, P18
[2]   Temporal activity of plasminogen activators and matrix metalloproteinases during cutaneous wound repair [J].
Arumugam, S ;
Jang, YC ;
Chen-Jensen, C ;
Gibran, NS ;
Isik, FF .
SURGERY, 1999, 125 (06) :587-593
[3]  
Berg S, 1969, Munch Med Wochenschr, V111, P1185
[4]   IMMUNOHISTOCHEMICAL ANALYSIS OF MARKERS FOR DIFFERENT MACROPHAGE PHENOTYPES AND THEIR USE FOR A FORENSIC WOUND AGE ESTIMATION [J].
BETZ, P ;
TUBEL, J ;
EISENMENGER, W .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 1995, 107 (04) :197-200
[5]   Affinity cytochemistry analysis of mast cells in skin lesions: a possible tool to assess the timing of lesions after death [J].
Bonelli, A ;
Bacci, S ;
Norelli, GA .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2003, 117 (06) :331-334
[6]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P159
[7]   PLASMINOGEN ACTIVATORS, TISSUE DEGRADATION, AND CANCER [J].
DANO, K ;
ANDREASEN, PA ;
GRONDAHLHANSEN, J ;
KRISTENSEN, P ;
NIELSEN, LS ;
SKRIVER, L .
ADVANCES IN CANCER RESEARCH, 1985, 44 :139-266
[8]   SYNTHESIS OF PROSTAGLANDIN-E BY PERITONEAL-MACROPHAGES FROM NZB/W MICE [J].
DOREDUFFY, P ;
GUHA, A ;
ROTHMAN, BL ;
ZURIER, RB .
LIFE SCIENCES, 1988, 42 (26) :2669-2676
[9]   UROKINASE-TYPE AND TISSUE-TYPE PLASMINOGEN ACTIVATORS IN KERATINOCYTES DURING WOUND REEPITHELIALIZATION INVIVO [J].
GRONDAHLHANSEN, J ;
LUND, LR ;
RALFKIAER, E ;
OTTEVANGER, V ;
DANO, K .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1988, 90 (06) :790-795
[10]  
GRULICHHENN J, 1989, Z KARDIOL, V78, P25