Down-Regulation of Stat3 Decreases Invasion Activity and Induces Apoptosis of Human Glioma Cells

被引：43

作者：

Chen, Fuxue ^{[1
]}

Xu, Yaming ^{[1
]}

Luo, Youqun ^{[1
]}

Zheng, Da ^{[1
]}

Song, Yichao ^{[1
]}

Yu, Kangkang ^{[1
]}

Li, Hong ^{[1
]}

Zhang, Li ^{[1
]}

Zhong, Weiwei ^{[1
]}

Ji, Yonghua ^{[1
]}

机构：

[1] Shanghai Univ, Mol Biol Lab, Sch Life Sci, Shanghai 200436, Peoples R China

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2010年 / 40卷 / 03期

关键词：

Glioma; Stat3; RNAi; Invasion; Apoptosis; SUPPRESSES GROWTH; EXPRESSION; ACTIVATION; GENE; CANCER; PROLIFERATION; EPIDEMIOLOGY; INHIBITION; KNOCKDOWN; SURVIVIN;

D O I：

10.1007/s12031-009-9323-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gliomas are the most common type of primary brain tumors. Despite the improvement in current treatments for gliomas, including surgical resection, radiation, and chemotherapy, there has been very little progress in curing this kind of disease. Stat3 is a member of signal transducer and activator of transcription family. It plays an important role in regulating cell survival, invasion, and apoptosis. This study investigated the influence of low-level expression of Stat3 on invasion and apoptosis in U251 cells. Our data showed that Stat3 is constitutively expressed in human gliomas cell line U251. The invasion activity in U251 cells was weakened and the apoptosis in U251 cells was induced after down-regulation of Stat3. In addition, down-regulation of Stat3 can suppress the expression of MMP-2, Bcl-xL and survivin but not 67LR. These results further indicate that Stat3 plays a key role in the invasion and apoptosis of human glioma cell line U251.

引用

页码：353 / 359

页数：7

共 33 条

[1] A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma [J].

Ambrosini, G ;

Adida, C ;

Altieri, DC .

NATURE MEDICINE, 1997, 3 (08) :917-921

[2] STATs in oncogenesis [J].

Bowman, T ;

Garcia, R ;

Turkson, J ;

Jove, R .

ONCOGENE, 2000, 19 (21) :2474-2488

[3] Stat proteins and oncogenesis [J].

Bromberg, J .

JOURNAL OF CLINICAL INVESTIGATION, 2002, 109 (09) :1139-1142

[4] Stat3 as an oncogene [J].

Bromberg, JF ;

Wrzeszczynska, MH ;

Devgan, G ;

Zhao, YX ;

Pestell, RG ;

Albanese, C ;

Darnell, JE .

CELL, 1999, 98 (03) :295-303

[5] Constitutive activation of Stat3 signaling confers resistance to apoptosis in human U266 myeloma cells [J].

Catlett-Falcone, R ;

Landowski, TH ;

Oshiro, MM ;

Turkson, J ;

Levitzki, A ;

Savino, R ;

Ciliberto, G ;

Moscinski, L ;

Fernández-Luna, JL ;

Nuñez, G ;

Dalton, WS ;

Jove, R .

IMMUNITY, 1999, 10 (01) :105-115

[6] Down-regulation of 67LR reduces the migratory activity of human glioma cells in vitro [J].

Chen, F. X. ;

Qian, Y. R. ;

Duan, Y. H. ;

Ren, W. W. ;

Yang, Y. ;

Zhang, C. C. ;

Qiu, Y. M. ;

Ji, Y. H. .

BRAIN RESEARCH BULLETIN, 2009, 79 (06) :402-408

[7] Inhibition of AF116909 gene expression enhances the differentiation of neural stem cells [J].

Chen, FX ;

Guo, QG ;

Yang, Y ;

Song, HS ;

Wen, TQ .

NEUROLOGICAL RESEARCH, 2005, 27 (05) :557-561

[8] STATs and gene regulation [J].

Darnell, JE .

SCIENCE, 1997, 277 (5332) :1630-1635

[9] Down-regulation of signal transducer and activator of transcription 3 expression using vector-based small interfering RNAs suppresses growth of human prostate tumor in vivo [J].

Gao, LF ;

Zhang, L ;

Hu, JD ;

Li, F ;

Shao, YT ;

Zhao, D ;

Kalvakolanu, DV ;

Kopecko, DJ ;

Zhao, XJ ;

Xu, DQ .

CLINICAL CANCER RESEARCH, 2005, 11 (17) :6333-6341

[10] Blockage of the STAT3 signaling pathway with a decoy oligonucleotide suppresses growth of human malignant glioma cells [J].

Gu, Jinhai ;

Li, Gang ;

Sun, Tao ;

Su, Yuhang ;

Zhang, Xulong ;

Shen, Jie ;

Tian, Zhigang ;

Zhang, Jian .

JOURNAL OF NEURO-ONCOLOGY, 2008, 89 (01) :9-17

← 1 2 3 4 →