In vivo analysis of the Drosophila light-sensitive channels, TRP and TRPL

被引：153

作者：

Reuss, H ^{[1
]}

Mojet, MH ^{[1
]}

Chyb, S ^{[1
]}

Hardie, RC ^{[1
]}

机构：

[1] Univ Cambridge, Dept Anat, Cambridge CB2 3DY, England

来源：

NEURON | 1997年 / 19卷 / 06期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/S0896-6273(00)80416-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have tested the proposal that the right-sensitive conductance in Drosophila is composed of two independent components by comparing the wild-type conductance with that in mutants lacking one or the other of the putative light-sensitive channel subunits, TRP and TRPL. For a wide range of cations, ionic permeability ratios in wild type were always intermediate between those of trp and trpl mutants. Effective channel conductances derived by noise analysis in wild type were again intermediate (17 pS; c.f. 35 pS in trp and 4 pS in trpl) and also showed a complex voltage dependence, which was quantitatively explained by the summation of TRPL and TRP channels after taking their different reversal potentials into account. Although La3+ partially blocked the light response in wild-type photoreceptors, it increased the effective single channel conductance. The results indicate that the wild-type light-activated conductance is composed of two separate channels, with the properties of TRP-and TRPL-dependent channels as determined in the respective mutants.

引用

页码：1249 / 1259

页数：11

共 37 条

[1] TRP is cracked but is CRAC TRP? [J].