Are LOD and LOQ Reliable Parameters for Sensitivity Evaluation of Spectroscopic Methods?

被引:30
作者
Ershadi, Saba [1 ,2 ,3 ]
Shayanfar, Ali [3 ,4 ]
机构
[1] Tabriz Univ Med Sci, Biotechnol Res Ctr, Tabriz, Iran
[2] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran
[3] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran
[4] Tabriz Univ Med Sci, Pharmaceut Anal Res Ctr, Tabriz, Iran
关键词
FLUORESCENCE METHOD;
D O I
10.5740/jaoacint.17-0363
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The limit of detection (LOD) and the limit of quantification (LOQ) are common parameters to assess the sensitivity of analytical methods. In this study, the LOD and LOQ of previously reported terbium sensitized analysis methods were calculated by different methods, and the results were compared with sensitivity parameters [lower limit of quantification (LLOQ)] of U.S. Food and Drug Administration guidelines. The details of the calibration curve and standard deviation of blank samples of three different terbium-sensitized luminescence methods for the quantification of mycophenolic acid, enrofloxacin, and silibinin were used for the calculation of LOD and LOQ. A comparison of LOD and LOQ values calculated by various methods and LLOQ shows a considerable difference. The significant difference of the calculated LOD and LOQ with various methods and LLOQ should be considered in the sensitivity evaluation of spectroscopic methods.
引用
收藏
页码:1212 / 1213
页数:2
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