Effects of icariin on cytokine-induced ankylosing spondylitis with fibroblastic osteogenesis and its molecular mechanism

被引：2
作者：
Jia, Chunrong [1 ]
Liu, Hongxiao [2 ]
Li, Min [3 ]
Wu, Zhikui [3 ]
Feng, Xinghua [2 ]
机构：
[1] Capital Med Univ, Beijing Tiantan Hosp, Dept Tradit Chinese Med, Beijing 100050, Peoples R China
[2] Chinese Acad Tradit Chinese Med, Dept Rheumatol, Guanganmen Hosp, Beijing 100053, Peoples R China
[3] China Acad Tradit Chinese Med, Dept Mol Biol, Guanganmen Hosp, Beijing 100053, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2014年 / 7卷 / 12期
关键词：
Ankylosing spondylitis; icariin; cytokine; ossification; fibroblast; BONE MORPHOGENETIC PROTEIN-2; EXPRESSION; TRANSCRIPTION; OSTEOCALCIN; OSTEOBLAST; DISEASE; DRUGS; CELLS; BETA;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of this study is to explore the effects of icariin on cytokine induced ankylosing spondylitis fibroblast osteogenesis type expression and its molecular mechanism. The normal fibroblasts were collected as normal control group, and the fibroblasts of hip joint capsule of AS patients were collected, which were respectively added in fetal bovine serum (group AS), fetal bovine serum and cytokines (BMP-2+TGF-beta 1) (group AS), and cell factor solution (icariin group), and observed of the osteogenic expression of fibroblast, to evaluate the impact of Icariin on it. The ALP activity, the content of collagen, osteocalcin content and cbfa1mRNA and OCmRNA of fibroblast of AS group increased compared to the normal control group and AS control group (P < 0.01), indicating that icariin can significantly inhibit the above changes (P < 0.01). Icariin can inhibit fibroblast further osteogenic differentiation through inhibiting the effect of cytokines on the fibroblast osteogenesis type markers and osteogenic gene expression and osteogenic differentiation.
引用
收藏
页码：9104 / 9109
页数：6
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