Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs

被引:13
作者
Rangel, Giselle [1 ,3 ]
Barcena, Juan [1 ]
Moreno, Noelia [1 ,4 ]
Mata, Carlos P. [2 ,5 ]
Caston, Jose R. [2 ]
Alejo, Ali [1 ]
Blanco, Esther [1 ]
机构
[1] CSIC, INIA, CISA, Madrid 28130, Spain
[2] CSIC, Ctr Nacl Biotecnol, Dept Struct Macromol, Madrid 28049, Spain
[3] Inst Invest Cient & Serv Alta Tecnol INDICASAT AI, City Of Knowledge 084301103, Panama
[4] Consejeria Agr, CFMR Moraleja, Junta De Extremadura 10840, Caceres, Spain
[5] Univ Leeds, Fac Biol Sci, Sch Mol & Cellular Biol, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
关键词
virus-like particles (VLPs); chimeric VLPs; nanoparticles; vaccine platform; RHDV; FMDV; B-cell epitope; T-cell epitope; immune response; pig; CAPSID PROTEIN; MOLECULAR SWITCH; IMMUNE-RESPONSE; RESEARCH UPDATE; VACCINE; SWINE; INDUCE;
D O I
10.3390/vaccines9050470
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Currently there is a clear trend towards the establishment of virus-like particles (VLPs) as a powerful tool for vaccine development. VLPs are tunable nanoparticles that can be engineered to be used as platforms for multimeric display of foreign antigens. We have previously reported that VLPs derived from rabbit hemorrhagic disease virus (RHDV) constitute an excellent vaccine vector, capable of inducing specific protective immune responses against inserted heterologous T-cytotoxic and B-cell epitopes. Here, we evaluate the ability of chimeric RHDV VLPs to elicit immune response and protection against Foot-and-Mouth disease virus (FMDV), one of the most devastating livestock diseases. For this purpose, we generated a set of chimeric VLPs containing two FMDV-derived epitopes: a neutralizing B-cell epitope (VP1 (140-158)) and a T-cell epitope [3A (21-35)]. The epitopes were inserted joined or individually at two different locations within the RHDV capsid protein. The immunogenicity and protection potential of the chimeric VLPs were analyzed in the mouse and pig models. Herein we show that the RHDV engineered VLPs displaying FMDV-derived epitopes elicit a robust neutralizing immune response in mice and pigs, affording partial clinical protection against an FMDV challenge in pigs.
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页数:22
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