2-D RP/RPLC method to separate components in Fructus schisandrae chinensis

被引：12

作者：

Jin, Gaowa ^{[1
,2
]}

Dai, Yuntao ^{[3
]}

Feng, Jiatao ^{[1
]}

Qin, Xuemei ^{[3
]}

Xue, Xingya ^{[1
]}

Zhang, Feifang ^{[1
,4
]}

Liang, Xinmiao ^{[1
,4
]}

机构：

[1] E China Univ Sci & Technol, Dalian Inst Chem Phys, Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China

[2] Chinese Acad Fishery Sci, E China Sea Fishery Res Inst, Shanghai, Peoples R China

[3] Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan, Peoples R China

[4] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2010年 / 33卷 / 4-5期

基金：

国家杰出青年科学基金;

关键词：

beta-CD column; Fructus schisandrae chinensis; Traditional Chinese medicine; 2-D RP/RPLC; 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY; HYDROPHILIC INTERACTION CHROMATOGRAPHY; RP-RP-HPLC; MASS-SPECTROMETRY; QUANTITATIVE-ANALYSIS; PHASE; CYCLODEXTRIN; PERFORMANCE; SYSTEM; CHEMISTRY;

D O I：

10.1002/jssc.200900563

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An off-line 2-D RP/RPLC method was developed to separate components in extracts of Fructus schisandrae chinensis, which is a traditional Chinese medicine. In this method, Click beta-CD stationary phase was proposed for constructing 2-D RP/RPLC system combined with C18 column. beta-CD column showed great potential to separate compounds in traditional Chinese medicines under RP mode. Collected fractions from Click beta-CD column used in first dimension were resolved into many additional components, indicating that the 2-D LC system based on Click beta-CD and C18 phases is orthogonal. The mobile phase was same on both columns. This 2-D LC method facilitates us not only to separate components that cannot be resolved by unidimensional chromatography, but also to achieve much more efficient detection of minor sample components.

引用

页码：564 / 569

页数：6

共 37 条

[1] Practice and mechanism of HPLC oligosaccharide separation with a cyclodextrin bonded phase [J].