Systemic RNAi in Caenorhabditis elegans

被引：36

作者：

Hunter, C. P. ^{[1
]}

Winston, W. M. ^{[1
]}

Molodowitch, C. ^{[1
]}

Feinberg, E. H. ^{[1
]}

Shih, J. ^{[1
]}

Sutherlin, M. ^{[1
]}

Wright, A. J. ^{[1
]}

Fitzgerald, M. C. ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

来源：

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY | 2006年 / 71卷

关键词：

D O I：

10.1101/sqb.2006.71.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference (RNAi) in Caenorhabditis elegans induced by ingestion or injection of double-stranded RNA (dsRNA) spreads throughout the organism and is even transmitted to the progeny. We have identified two proteins required for spreading of RNAi, SID-1 and SID-2, whose structure, subcellular localization, and expression pattern have been informative for how dsRNA can be transported into and between cells. SID-1 is a transmembrane protein that functions as a pore or channel that transports dsRNA into and Out of cells. Proteins homologous to SID-1 are present in a wide range of invertebrate and vertebrate animals but are absent from plants. SID-2 is a small transmembrane protein that is expressed in the gut and localizes strongly to the luminal membrane where it appears to act as a receptor for uptake of dsRNA from the environment. Characterization of SID-2 activity in a variety of Caenorhabditis nematodes indicates that C. elegans SID-2 may have a novel activity.

引用

页码：95 / 100

页数：6

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