Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa

被引：181
|
作者：
Taura, Futoshi [1 ]
Sirikantaramas, Supaart
Shoyama, Yoshinari
Yoshikai, Kazuyoshi
Shoyama, Yukihiro
Morimoto, Satoshi
机构：
[1] Kyushu Univ, Grad Sch Pharmaceut Sci, Fukuoka 8128582, Japan
[2] Chiba Univ, Grad Sch Pharmaceut Sci, Chiba 2638522, Japan
来源：
FEBS LETTERS | 2007年 / 581卷 / 16期
关键词：
cannabidiolic-acid synthase; cannabinoid; biosynthesis; molecular cloning; Cannabis saliva;
D O I：
10.1016/j.febslet.2007.05.043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：2929 / 2934
页数：6
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