Single L-type calcium channels in smooth muscle cells from resistance arteries of spontaneously hypertensive rats

被引:57
|
作者
Ohya, Y [1 ]
Tsuchihashi, T [1 ]
Kagiyama, S [1 ]
Abe, I [1 ]
Fujishima, M [1 ]
机构
[1] Kyushu Univ, Fac Med, Dept Internal Med 2, Higashi Ku, Fukuoka 81282, Japan
关键词
muscle; smooth; vascular; electrophysiology; calcium channels; vascular resistance; hypertension; genetic;
D O I
10.1161/01.HYP.31.5.1125
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
The amplitude of the whole-cell L-type Ca2+ channel current recorded from vascular smooth muscle cells is reportedly greater in spontaneously hypertensive rats (SHR) than in Wistar-Kyoto rats (WKY), However, no study has examined properties of single Ca2+ channels in arterial cells from these strains. To further test the hypothesis that activation of L-type Ca2+ channels in arterial smooth muscle cells would be enhanced in SHR, we recorded single Ca2+ channel currents in resistance mesenteric artery cells from SHR and WKY (8 to 9 weeks of age) using a cell-attached patch clamp technique, With 50 mmol/L Ba2+ in the recording pipette, the depolarizing pulse from a holding potential of -40 mV evoked the single L-type Ca2+ channel current. Opening of the single channels was more frequent in cells from SHR than from WKY. Single-channel conductance (20 pS) and open time (1 ms at 0 mV) did not differ in the two strains, The results suggest that an increased amplitude of the whole-cell current can be attributed to the enhanced opening of single Ca2+ channels in the arterial smooth muscle cells from SHR compared with WKY.
引用
收藏
页码:1125 / 1129
页数:5
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