Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent

被引：52
|
作者：
Zambare, V. P. [1 ]
Nilegaonkar, S. S. [1 ]
Kanekar, P. P. [1 ]
机构：
[1] Agharkar Res Inst, Microbial Sci Div, Pune 411004, Maharashtra, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 23卷 / 11期
关键词：
Bacillus cereus; alkaline protease; dehairing; buffalo hide; leather processing;
D O I：
10.1007/s11274-007-9402-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The present investigation describes microbial production of an alkaline protease and its use in dehairing of buffalo hide. Bacillus cereus produced extracellular protease when grown on a medium containing starch, wheat bran and soya flour (SWS). The ammonium sulphate precipitated (ASP) enzyme was applied for dehairing of buffalo hide. Microscopic observation of longitudinal section of buffalo hide revealed that the epidermis was completely removed and hair was uprooted leaving empty follicles in the hide. The ASP enzyme was stable for one month at ambient temperature between 25-35 degrees C. Enzymatic dehairing may be a promising shift towards an environment-friendly leather processing method.
引用
收藏
页码：1569 / 1574
页数：6
相关论文
共 50 条
  • [21] Enhanced production of alkaline protease by Neocosmospora sp. N1 using custard apple seed powder as inducer and its application for stain removal and dehairing
    Matkawala, Fatema
    Nighojkar, Sadhana
    Kumar, Anil
    Nighojkar, Anand
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2019, 21
  • [22] Production of moderately halotolerant, SDS stable alkaline protease from Bacillus cereus MTCC 6840 isolated from lake Nainital, Uttaranchal state, India
    Joshi, Gopal K.
    Kumar, Sarvesh
    Sharma, Vinay
    BRAZILIAN JOURNAL OF MICROBIOLOGY, 2007, 38 (04) : 773 - 779
  • [23] Production of alkaline protease by rhizospheric Bacillus cereus HP_RZ17 and Paenibacillus xylanilyticus HP_RZ19
    H. P. Jadhav
    M. S. Sonawane
    M. H. Khairnar
    R. Z. Sayyed
    Environmental Sustainability, 2020, 3 (1) : 5 - 13
  • [24] Production and eco friendly application of alkaline protease from Bacillus amyloliquifaciens sp1
    Guleria, Shiwani
    Walia, Abhishek
    Chauhan, Anjali
    Shirkot, C. K.
    INDIAN JOURNAL OF BIOTECHNOLOGY, 2018, 17 (03): : 448 - 458
  • [25] Production, Partial Purification and Optimization of Oilseed-Based Protease and its Application as an Efficient Eco-Friendly Alternative for Destaining and Dehairing Process
    Akhter, Kulsoom
    Kiani, Hina Akbar
    Ghous, Tahseen
    Rasheed, Aamir
    Gillani, Humaira
    Akhtar, Tasleem
    WASTE AND BIOMASS VALORIZATION, 2024, 15 (07) : 3929 - 3938
  • [26] Overexpression of Bacillus circulans alkaline protease in Bacillus subtilis and its potential application for recovery of protein from soybean dregs
    Chen, Hao
    Wu, Jie
    Huang, Xiaodan
    Feng, Xuzhong
    Ji, Hongwu
    Zhao, Liangzhong
    Wang, Jianrong
    FRONTIERS IN MICROBIOLOGY, 2022, 13
  • [27] Production of alkaline protease from Streptomyces gulbargensis and its application in removal of blood stains
    Vishalakshi, N.
    Lingappa, K.
    Amena, S.
    Prabhakar, M.
    Dayanand, A.
    INDIAN JOURNAL OF BIOTECHNOLOGY, 2009, 8 (03): : 280 - 285
  • [28] Optimization of fermentation conditions for production of neutral metalloprotease by Bacillus subtilis SCK6 and its application in goatskin-dehairing
    He, Fuming
    Jin Chao
    Yang, Dandan
    Zhang, Xinqing
    Yang, Chuanlun
    Xu, Zeping
    Tian Jiewei
    Tian Yongqiang
    PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2022, 52 (07) : 789 - 799
  • [29] Genetic improvement of a locally isolated Bacillus cereus AUST-7 strain via mutagenesis for alkaline protease production and its optimization using artificial neural network (ANN)
    Ullah, Najeeb
    Mujaddad-ur-Rehman, Malik
    Nadeem, Muhammad
    Nelofer, Rubina
    Sarwar, Abid
    Aziz, Tariq
    Alharbi, Metab
    Alshammari, Abdulrahman
    Alasmari, Abdullah F.
    BIOMASS CONVERSION AND BIOREFINERY, 2025, 15 (02) : 2453 - 2463
  • [30] Effect of bioprocess parameters on alkaline protease production by locally isolated Bacillus cereus AUST-7 using tannery waste in submerged fermentation
    Ullah, Najeeb
    Mujaddad-ur-Rehman, Malik
    Sarwar, Abid
    Nadeem, Muhammad
    Nelofer, Rubina
    Irfan, Muhammad
    Idrees, Muhammad
    Ali, Urooj
    Naz, Sumaira
    Aziz, Tariq
    BIOMASS CONVERSION AND BIOREFINERY, 2023, 14 (18) : 22977 - 22987
12345