Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent

被引：52
|
作者：
Zambare, V. P. [1 ]
Nilegaonkar, S. S. [1 ]
Kanekar, P. P. [1 ]
机构：
[1] Agharkar Res Inst, Microbial Sci Div, Pune 411004, Maharashtra, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 23卷 / 11期
关键词：
Bacillus cereus; alkaline protease; dehairing; buffalo hide; leather processing;
D O I：
10.1007/s11274-007-9402-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The present investigation describes microbial production of an alkaline protease and its use in dehairing of buffalo hide. Bacillus cereus produced extracellular protease when grown on a medium containing starch, wheat bran and soya flour (SWS). The ammonium sulphate precipitated (ASP) enzyme was applied for dehairing of buffalo hide. Microscopic observation of longitudinal section of buffalo hide revealed that the epidermis was completely removed and hair was uprooted leaving empty follicles in the hide. The ASP enzyme was stable for one month at ambient temperature between 25-35 degrees C. Enzymatic dehairing may be a promising shift towards an environment-friendly leather processing method.
引用
收藏
页码：1569 / 1574
页数：6
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