The size of cell-free mitochondrial DNA in blood is inversely correlated with tumor burden in cancer patients

被引：29

作者：

An, Qin ^{[1
]}

Hu, Youjin ^{[1
]}

Li, Qingjiao ^{[2
]}

Chen, Xufeng ^{[2
,3
]}

Huang, Jiaoti ^{[2
,3
]}

Pellegrini, Matteo ^{[4
]}

Zhou, Xianghong Jasmine ^{[2
]}

Rettig, Matthew ^{[5
]}

Fan, Guoping ^{[1
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

[3] Duke Univ, Sch Med, Dept Pathol, Durham, NC 27710 USA

[4] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Urol, Los Angeles, CA 90095 USA

来源：

PRECISION CLINICAL MEDICINE | 2019年 / 2卷 / 03期

基金：

美国国家卫生研究院;

关键词：

circulating cell-free DNA (cfDNA); tumor burden; cancer progression; liquid biopsy;

D O I：

10.1093/pcmedi/pbz014

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Circulating cell-free DNAs (cfDNAs) are fragmented DNA molecules released into the blood by cells. Previous studies have suggested that mitochondria-originated cfDNA fragments (mt-cfDNAs) in cancer patients are more fragmented than those from healthy controls. However, it is still unknown where these short mt-cfDNAs originate, and whether the length of mt-cfDNAs can be correlated with tumor burden and cancer progression. In this study, we first performed whole-genome sequencing analysis (WGS) of cfDNAs from a human tumor cell line-xenotransplantation mouse model and found that mt-cfDNAs released from transplanted tumor cells were shorter than the mouse counterpart. We next analyzed blood cfDNA samples from hepatocellular carcinoma and prostate cancer patients and found that mt-cfDNA lengths were inversely related to tumor size as well as the concentration of circulating tumor DNA. Our study suggested that monitoring the size of mt-cfDNAs in cancer patients would be a useful way to estimate tumor burden and cancer progression.

引用

页码：131 / 139

页数：9

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