Effects of different extraction techniques on physicochemical properties and activities of polysaccharides from comfrey (Symphytum officinale L.) root

被引：101

作者：

Chen, Shilun ^{[1
]}

Shang, Hongmei ^{[1
,2
]}

Yang, Junyan ^{[1
]}

Li, Ran ^{[1
]}

Wu, Hongxin ^{[3
]}

机构：

[1] Jilin Agr Univ, Coll Anim Sci & Technol, 2888 Xin Cheng St, Changchun 130118, Jilin, Peoples R China

[2] Jilin Agr Univ, Key Lab Anim Nutr & Feed Sci Jilin Prov, Changchun 130118, Jilin, Peoples R China

[3] Grassland Res Inst CAAS, Hohhot 010010, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2018年 / 121卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Symphytum officinale L. polysaccharides; Extraction techniques; Antioxidant activity; Antidiabetic activity; HOHENBUEHELIA-SEROTINA POLYSACCHARIDES; WATER-SOLUBLE POLYSACCHARIDES; ANTIOXIDANT ACTIVITY; ASSISTED EXTRACTION; IN-VITRO; BIOLOGICAL-ACTIVITIES; ENZYMATIC EXTRACTION; BLACK-CURRANT; ALPHA-AMYLASE; DRYING METHOD;

D O I：

10.1016/j.indcrop.2018.04.063

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Comfrey (Symphytum officinale L.) has long been used in folk medicine due to its effects of anti-inflammatory and acesodyne. The present study was designed to evaluate the effects of extraction techniques on the physicochemical properties and activities of polysaccharides from comfrey root (CRPs). CRPs were extracted by using four methods including hot water extraction (HW), ultrasonic assistance extraction (UA), enzyme assistance extraction (EA) and enzyme-ultrasonic assistance extraction (EUA). The results showed that CRPs extracted by EUA method (EUA-CRPs) had the highest extraction yield of 24.51%. HPLC analysis presented that the monosaccharide compositions of the four CRPs were indentical, but the monosaccharide content was significantly different. EUA-CRPs had better antioxidant activity, which might be related to its smaller molecular weight and higher content of uronic acid. EUA-CRPs exhibited notable a-glucosidase inhibition activity. The results suggested that enzyme-ultrasonic assistance technology was a good way to extract polysaccharides from comfrey root.

引用

页码：18 / 25

页数：8

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