Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution

被引：22

作者：

Song, Yanting ^{[1
,2
]}

Takatsuki, Katsuya ^{[3
]}

Sekiguchi, Tetsushi ^{[3
]}

Funatsu, Takashi ^{[1
]}

Shoji, Shuichi ^{[3
]}

Tsunoda, Makoto ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Tokyo, Japan

[2] Hainan Univ, Coll Marine Sci, Key Lab Trop Biol Resources, Minister Educ, Haikou, Peoples R China

[3] Waseda Univ, Nanosci & Nanoengn, Tokyo, Japan

来源：

AMINO ACIDS | 2016年 / 48卷 / 07期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

Microfluidics; 4-Fluoro-7-nitro-2,1,3-benzoxadiazole; Fluorescence; Quantitative determination; Amino acids; LIQUID-CHROMATOGRAPHY; AMINO-ACIDS; FLUORESCENCE DETECTION; PERFORMANCE; PHENYLKETONURIA; FABRICATION; DISPERSION; SAMPLES; HPLC;

D O I：

10.1007/s00726-016-2248-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.

引用

收藏

页码：1731 / 1735

页数：5

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