Fusarium verticillioides Strains Isolated from Corn Feed: Characterization by Fumonisin Production and RAPD Fingerprinting

被引：5

作者：

Sataque Ono, Elisabete Yurie ^{[1
]}

Pelegrinelli Fungaro, Maria Helena ^{[2
]}

Sofia, Silvia Helena ^{[2
]}

Miguel, Tatiana de Avila ^{[1
]}

Sugiura, Yoshitsugu ^{[3
]}

Hirooka, Elisa Yoko ^{[4
]}

机构：

[1] Univ Estadual Londrina, Dept Bioquim & Biotecnol, BR-86051990 Londrina, PR, Brazil

[2] Dept Biol Geral, BR-86051990 Londrina, PR, Brazil

[3] Kobe Inst Hlth, Dept Food Chem, Chuo Ku, Kobe, Hyogo 6500046, Japan

[4] Univ Estadual Londrina, Dept Ciencia & Tecnol Alimentos, BR-86051990 Londrina, PR, Brazil

来源：

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2010年 / 53卷 / 04期

关键词：

Fumonisin; Fusarium verticillioides; genetic variability; RAPD; GIBBERELLA-FUJIKUROI; GENETIC DIVERSITY; MONILIFORME; DNA; MAIZE; POLYMORPHISMS; GRAMINEARUM; CEREALS; SECTION; MARKERS;

D O I：

10.1590/S1516-89132010000400026

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB(1) and FB(2) with levels ranging from 2.41 to 3996.36 mu g/g, and from 1.18 to 1209.91 mu g/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 mu g/g), eight as intermediate (>1289.84 to 3772.44 mu g/g) and four strains as high (>3772.44 mu g/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F. verticillioides strains, there was no clear association between these makers and fumonisin production.

引用

页码：953 / 960

页数：8

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