Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system

被引：1
|
作者：
Farooq, Umer [1 ,2 ]
Notani, Dimple [1 ]
机构：
[1] Tata Inst Fundamental Res, Natl Ctr Biol Sci, Bangalore 560065, Karnataka, India
[2] Univ Transdisciplinary Hlth Sci & Technol, IVRI Rd, Bangalore 560064, Karnataka, India
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 04期
关键词：
CRISPR; Gene Expression; Genomics; Molecular Biology; Single Cell;
D O I：
10.1016/j.xpro.2021.100857
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The CRISPR/Cas9 system is a powerful tool for genome editing and is adaptable for a wide range of applications. Here, we have put together a step-by-step protocol for generating knockout cell lines (coding or non-coding region) using CRISPR/Cas9 tool. The protocol below has been tested on adherent cell lines such as HeLa and MCF7. However, it may easily be adapted to other adherent cell lines with minor variations.For complete details on the use and execution of this protocol, please refer to Farooq et al. (2021).
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页数：17
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