Antimicrobial responses of peripheral and central nervous system glia against Staphylococcus aureus

被引:4
作者
Choudhury, Indra N. [1 ,2 ]
Chacko, Anu [1 ,2 ]
Delbaz, Ali [1 ,2 ]
Chen, Mo [1 ,2 ]
Basu, Souptik [1 ,2 ]
St John, James A. [1 ,2 ,3 ]
Huygens, Flavia [4 ]
Ekberg, Jenny A. K. [1 ,2 ,3 ]
机构
[1] Griffith Univ, Clem Jones Ctr Neurobiol & Stem Cell Res, Nathan, Qld, Australia
[2] Griffith Univ, Menzies Hlth Inst Queensland, Southport, Qld 4222, Australia
[3] Griffith Univ, Griffith Inst Drug Discovery, Nathan, Qld, Australia
[4] Queensland Univ Technol, Fac Hlth, Ctr Immunol & Infect Control, Sch Biomed Sci, Brisbane, Qld, Australia
关键词
OLFACTORY ENSHEATHING CELLS; TUMOR-NECROSIS-FACTOR; SPINAL-CORD; BACTERIAL CHALLENGE; RECEPTOR; PHAGOCYTOSIS; INFLAMMATION; EXPRESSION; CYTOKINES; REPAIR;
D O I
10.1038/s41598-021-90252-0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Staphylococcus aureus infections of the central nervous system are serious and can be fatal. S. aureus is commonly present in the nasal cavity, and after injury to the nasal epithelium it can rapidly invade the brain via the olfactory nerve. The trigeminal nerve constitutes another potential route of brain infection. The glia of these nerves, olfactory ensheathing cells (OECs) and trigeminal nerve Schwann cells (TgSCs), as well as astrocytes populating the glia limitans layer, can phagocytose bacteria. Whilst some glial responses to S. aureus have been studied, the specific responses of different glial types are unknown. Here, we compared how primary mouse OECs, TgSCs, astrocytes and microglia responded to S. aureus. All glial types internalized the bacteria within phagolysosomes, and S. aureus-conjugated BioParticles could be tracked with subtle but significant differences in time-course of phagocytosis between glial types. Live bacteria could be isolated from all glia after 24 h in culture, and microglia, OECs and TgSCs exhibited better protection against intracellular S. aureus survival than astrocytes. All glial types responded to the bacteria by cytokine secretion. Overall, OECs secreted the lowest level of cytokines, suggesting that these cells, despite showing strong capacity for phagocytosis, have immunomodulatory functions that can be relevant for neural repair.
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页数:15
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