Comparison of assays to detect cytomegalovirus shedding in the semen of HIV-infected men

被引:8
作者
Diamond, C
Speck, C
Huang, ML
Corey, L
Coombs, RW
Krieger, JN
机构
[1] Univ Washington, Sch Publ Hlth, Seattle, WA 98195 USA
[2] Fred Hutchinson Canc Res Ctr, Program Infect Dis, Seattle, WA 98104 USA
[3] Univ Washington, Med Ctr, Dept Urol, Seattle, WA 98195 USA
[4] Univ Washington, Med Ctr, Dept Med & Lab Med, Seattle, WA 98195 USA
关键词
cytomegalovirus (CMV); HIV-1; semen; CD4+; homosexual men; viral infections;
D O I
10.1016/S0166-0934(00)00232-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We sought to determine the optimal assays for cytomegalovirus (CMV) shedding in semen. Over a 2-month period, 149 HIV-1-infected men who have sex with men each provided up to three semen specimens. Specimens were tested for CMV by culture, rapid assay (shell vial) and polymerase chain reaction (PCR). By culture, 30% of seminal plasma and 28% of seminal cell specimens grew CMV. By rapid assay, results were 38 and 33%, respectively. By PCR, 56% of seminal cell specimens demonstrated CMV: 20% in a single semen specimen; 33% in two specimens; and 34% in all three specimens. Overall, 69% of men had CMV detected by PCR in at least one seminal eel specimen. By quantitative PCR, 14% had ten, 14% had 100, 16% had 1000, and 12% had 10 000 copies in 6.25 mul of semen analyzed. Adjusting for initial CD4+ cell count, men with CMV shedding demonstrated by PCR at the first visit were approximately four times as likely to shed CMV at a subsequent visit (RR 4.28, CI: 2.30-7.95). CMV shedding was associated with decreased CD4+ cell counts in peripheral blood (P = 0.05), It is concluded that the PCR assay provided the greatest sensitivity among the three detection methods. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:185 / 191
页数:7
相关论文
共 31 条
[1]  
[Anonymous], 1992, MMWR-MORBID MORTAL W, V41, P1
[2]   COMPARISON OF MONOCLONAL-ANTIBODIES FOR RAPID DETECTION OF CYTOMEGALO-VIRUS IN SPIN-AMPLIFIED PLATE CULTURES [J].
ASHLEY, R ;
PETERSON, E ;
ABBO, H ;
GOLD, D ;
COREY, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (12) :2858-2860
[3]   A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS [J].
BOSHART, M ;
WEBER, F ;
JAHN, G ;
DORSCHHASLER, K ;
FLECKENSTEIN, B ;
SCHAFFNER, W .
CELL, 1985, 41 (02) :521-530
[4]   CYTOMEGALOVIRUS-INFECTION IN HOMOSEXUAL MEN - RELATIONSHIP TO SEXUAL PRACTICES, ANTIBODY TO HUMAN-IMMUNODEFICIENCY-VIRUS, AND CELL-MEDIATED-IMMUNITY [J].
COLLIER, AC ;
MEYERS, JD ;
COREY, L ;
MURPHY, VL ;
ROBERTS, PL ;
HANDSFIELD, HH .
AMERICAN JOURNAL OF MEDICINE, 1987, 82 (03) :593-601
[5]   COAMPLIFIED POSITIVE CONTROL DETECTS INHIBITION OF POLYMERASE CHAIN-REACTIONS [J].
CONE, RW ;
HOBSON, AC ;
HUANG, MLW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (12) :3185-3189
[6]   Association between culturable human immunodeficiency virus type 1 (HIV-1) in semen and HIV-1 RNA levels in semen and blood: Evidence for compartmentalization of HIV-1 between semen and blood [J].
Coombs, RW ;
Speck, CE ;
Hughes, JP ;
Lee, W ;
Sampoleo, R ;
Ross, SO ;
Dragavon, J ;
Peterson, G ;
Hooton, TM ;
Collier, AC ;
Corey, L ;
Koutsky, L ;
Krieger, JN .
JOURNAL OF INFECTIOUS DISEASES, 1998, 177 (02) :320-330
[7]   IMMEDIATE-EARLY GENE REGION OF HUMAN CYTOMEGALOVIRUS TRANS-ACTIVATES THE PROMOTER OF HUMAN-IMMUNODEFICIENCY-VIRUS [J].
DAVIS, MG ;
KENNEY, SC ;
KAMINE, J ;
PAGANO, JS ;
HUANG, ES .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8642-8646
[8]   Loss of cytomegalovirus (CMV) viraemia following highly active antiretroviral therapy in the absence of specific anti-CMV therapy [J].
Deayton, J ;
Mocroft, A ;
Wilson, P ;
Emery, VC ;
Johnson, MA ;
Griffiths, PD .
AIDS, 1999, 13 (10) :1203-1206
[9]   THE RELATION OF CYTOMEGALOVIRUS AND EPSTEIN-BARR VIRUS-ANTIBODIES TO T-CELL SUBSETS IN HOMOSEXUALLY ACTIVE MEN [J].
DETELS, R ;
VISSCHER, BR ;
FAHEY, JL ;
SCHWARTZ, K ;
GREENE, RS ;
MADDEN, DL ;
SEVER, JL ;
GOTTLIEB, MS .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1984, 251 (13) :1719-1722
[10]   PERSISTENT CYTOMEGALOVIRUS-INFECTION OF SEMEN INCREASES RISK OF AIDS [J].
DETELS, R ;
LEACH, CT ;
HENNESSEY, K ;
LIU, ZY ;
VISSCHER, BR ;
CHERRY, JD ;
GIORGI, JV .
JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (04) :766-768