Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance

被引:0
作者
Deak, Eszter [1 ]
Etienne, Kizee A. [1 ]
Lockhart, Shawn R. [1 ]
Gade, Lalitha [1 ]
Chiller, Tom [1 ]
Balajee, S. Arunmozhi [1 ]
机构
[1] Ctr Dis Control & Prevent, Mycot Dis Branch, Atlanta, GA 30333 USA
关键词
Luminex; Candida; species identification; FLOW-CYTOMETRY; XMAP TECHNOLOGY; DNA PROBES; SYSTEM; YEASTS;
D O I
10.1139/W10-003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A Candida-specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.
引用
收藏
页码:348 / 351
页数:4
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