Brilliant cresyl blue as electroactive indicator in electrochemical DNA oligonucleotide sensors

被引：42

作者：

Hejazi, Mohammad Saeid ^{[2
,3
,4
]}

Raoof, Jahan-Bakhsh ^{[1
]}

Ojani, Reza ^{[1
]}

Golabi, Seyd Mehdi ^{[5
]}

Asl, Ezat Hamidi ^{[1
]}

机构：

[1] Mazandran Univ, Electroanalyt Chem Res Lab, Dept Analyt Chem, Fac Chem, Babol Sar, Iran

[2] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

[3] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[4] Tabriz Univ Med Sci, Pharmaceut Nanotechnol Res Ctr, Tabriz, Iran

[5] Tabriz Univ, Electroanalyt Chem Res Lab, Dept Analyt Chem, Fac Chem, Tabriz, Iran

来源：

BIOELECTROCHEMISTRY | 2010年 / 78卷 / 02期

关键词：

Brilliant cresyl blue; Carbon paste electrode; Differential pulse voltammetry; DNA biosensor; Electroactive label; HUMAN INTERLEUKINE-2 GENE; METHYLENE-BLUE; SPECTROPHOTOMETRIC DETERMINATION; DEVELOPMENTAL COMPETENCE; HYBRIDIZATION; BIOSENSOR; IMMOBILIZATION; CYSTEAMINE; GENOSENSOR; IMPEDANCE;

D O I：

10.1016/j.bioelechem.2009.09.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new electrochemical DNA biosensor is presented based on carbon past electrode (CPE) for immobilization and detection of short DNA sequences with brilliant cresyl blue (BCB) as electroactive label. The interaction of BCB with DNA is electrochemically detected and BCB displays different signals in the interaction to ssDNA and dsDNA and variation in the BCB signal represents the extent of hybridization at the electrode surface. The effect of solution pH on electrochemical behavior of BCB was investigated. Additionally, the effect of solution pH on BCB accumulation on the CPE was studied. Furthermore, experiments showed that the solution pH could influence the differential pulse voltammetry (DPV) signal of BCB accumulated on the electrode and the highest BCB signal was obtained in pH 7.00. The effect of electrochemical pretreatment of CPE on the ability of electrode in probe adsorption, BCB accumulation and conditions of probe immobilization including potential and time was investigated and optimum conditions were suggested. The peak currents of BCB were linearly related to the concentration of the target oligonucleotide sequence in the range of 1.0 x 10(-8) to 5.0 x 10(-6) M. The detection limit of this approach was 9.00 nM. The selectivity of the biosensor was studied using noncomplementary oligonucleotide. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：141 / 146

页数：6

共 39 条

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