Lipopolysaccharide-induced TNFα factor (LITAF) promotes inflammatory responses and activates apoptosis in zebrafish Danio rerio

被引：19

作者：

Chen, Kangyong ^{[1
,2
]}

Tian, Jiayin ^{[1
,2
]}

Wang, Junya ^{[1
,2
]}

Jia, Zhao ^{[1
,2
]}

Zhang, Qin ^{[1
,2
]}

Huang, Wenji ^{[1
,2
]}

Zhao, Xin ^{[1
,2
]}

Gao, Zhipeng ^{[3
]}

Gao, Qian ^{[1
,2
]}

Zou, Jun ^{[1
,2
,4
]}

机构：

[1] Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

[2] Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai, Peoples R China

[3] Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Coll Anim Sci & Technol, Changsha 410128, Peoples R China

[4] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China

来源：

GENE | 2021年 / 780卷

基金：

国家重点研发计划;

关键词：

Lipopolysaccharide-induced TNF? factor; TNF; Apoptosis; Inflammation; Zebrafish;

D O I：

10.1016/j.gene.2021.145487

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Lipopolysaccharide-induced TNF? factor (LITAF) is an important transcription factor which activates the transcription of TNF? and regulates cell apoptosis and inflammatory response. In the present study, a LITAF gene homologue was identified in zebrafish (Danio rerio) and was shown to be well conserved in the protein sequence, genomic organization and synteny with human LITAF. DrLITAF was constitutively expressed in tissues, with the highest expression detected in the gills. Its expression could be modulated by LPS, poly(I:C), and infection with Edwardsiella tarda, Aeromonus hydrophila and septicemia viremia of carp virus (SVCV). DrLITAF, when overexpressed, was shown to be located on the cellular membrane and nuclear membrane of HEK293T and ZF4 cells and was associated with the endoplasmic reticulum. Stimulation with LPS resulted in rapid translocation of DrLITAF into the nucleus. In addition, DrLITAF was able to induce cell apoptosis and the expression of caspase 3. The results demonstrate that DrLITAF is involved in the immune defence against bacterial and viral infection and plays a role in regulating inflammation and apoptosis.

引用

页数：8

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