Crystallization and preliminary X-ray diffraction studies of infectious bronchitis virus nonstructural protein 9

被引：1

作者：

Ma, Yanlin ^{[1
]}

Chen, Cheng ^{[2
]}

Wei, Lei ^{[2
]}

Yang, Qingzhu ^{[1
]}

Liao, Ming ^{[3
]}

Li, Xuemei ^{[1
]}

机构：

[1] Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China

[2] Tsinghua Univ, Struct Biol Lab, Beijing 100084, Peoples R China

[3] S China Agr Univ, Lab Avian Med, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2010年 / 66卷

基金：

中国国家自然科学基金;

关键词：

CORONAVIRUS; NSP9; REPLICATION; GENOME;

D O I：

10.1107/S174430911001417X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Avian infectious bronchitis virus (IBV), which causes respiratory disease in infected birds, belongs to coronavirus group 3. IBV encodes 15 nonstructural proteins (nsp2-nsp16) which play crucial roles in RNA transcription and genome replication. Nonstructural protein 9 (nsp9) has been identified as a protein that is essential to viral replication because of its single-stranded RNA-binding ability. The gene segment encoding IBV nsp9 has been cloned and expressed in Escherichia coli. The protein has been crystallized and the crystals diffracted X-rays to 2.44 angstrom resolution. They belonged to the cubic space group I432, with unit-cell parameters a = b = c = 123.4 angstrom, alpha = beta = gamma = 90 degrees. The asymmetric unit appeared to contain one molecule, with a solvent content of 62% (V-M = 3.26 angstrom(3) Da(-1)).

引用

页码：706 / 708

页数：3

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