Expression and biochemical characterization of the 1-HO-carotenoid methylase CrtF from Rhodobacter capsulatus

被引：12

作者：

Badenhop, F ^{[1
]}

Steiger, S ^{[1
]}

Sandmann, M ^{[1
]}

Sandmann, G ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Biosynth Grp, Inst Bot, D-60054 Frankfurt, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 222卷 / 02期

关键词：

acyclic 1-HO-carotenoid methylase; CrtF; Rhodobacter capsulatus; spheroidene biosynthesis; spirilloxanthin biosynthesis;

D O I：

10.1016/S0378-1097(03)00302-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In purple bacteria, acyclic 1-methoxy carotenoids like spheroidene or spirilloxanthin are essential components of the photosynthetic apparatus. One of the last steps of their biosynthesis involves O-methylation of the 1-hydroxy group. The 1-HO-carotenoid methylase CrtF from Rhodobacter capsulatus catalyzing this type of reaction was expressed in Escherichia coli in an active form. It was then purified by affinity chromatography and biochemically characterized. The enzymatic reaction depends on S-adenosylmethionine as the only cofactor. By complementation in E coli, the substrate specificity of the enzyme was determined. It could be shown that the enzyme converts not only all possible 1-hydroxy carotenoids in the spheroidene/1'-HO-spheroidene biosynthetic pathway of R. capsulatus but also carotenoid intermediates leading to the formation of spirilloxanthin in a pathway which is absent in R. capsulatus but present in related species. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：237 / 242

页数：6

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