Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons

被引：111

作者：

Wen, Hongxiu ^{[1
,2
]}

Lan, Xiqian ^{[2
]}

Zhang, Yuansong ^{[1
]}

Zhao, Tianfu ^{[1
]}

Wang, Yujun ^{[1
]}

Kajiura, Zenta ^{[1
]}

Nakagaki, Masao ^{[1
]}

机构：

[1] Shinshu Univ, Div Appl Biol, Fac Text Sci & Technol, Nagano 3868567, Japan

[2] Southwest Univ, Key Sericultural Lab Agr Minist, Chongqing 400716, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 04期

基金：

日本学术振兴会;

关键词：

Spider dragline silk; Transgenic silkworm; piggyBac; Cocoon proteins; Mechanical characteristic; EGG CASE SILK; MOLECULAR ARCHITECTURE; PIGGYBAC TRANSPOSON; SERICIN LAYER; PROTEINS; EXPRESSION; TRANSFORMATION; FIBROIN; FIBER; GENE;

D O I：

10.1007/s11033-009-9615-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spider dragline silk is a unique fibrous protein with a combination of tensile strength and elasticity, but the isolation of large amounts of silk from spiders is not feasible. In this study, we generated germline-transgenic silkworms (Bombyx mori) that spun cocoons containing recombinant spider silk. A piggyBac-based transformation vector was constructed that carried spider dragline silk (MaSp1) cDNA driven by the sericin 1 promoter. Silkworm eggs were injected with the vector, producing transgenic silkworms displaying DsRed fluorescence in their eyes. Genotyping analysis confirmed the integration of the MaSp1 gene into the genome of the transgenic silkworms, and silk protein analysis revealed its expression and secretion in the cocoon. Compared with wild-type silk, the recombinant silk displayed a higher tensile strength and elasticity. The results indicate the potential for producing recombinant spider silk in transgenic B. mori.

引用

页码：1815 / 1821

页数：7

共 31 条

[31] Fine organization of Bombyx mori fibroin heavy chain gene [J].

Zhou, CZ ;

Confalonieri, F ;

Medina, N ;

Zivanovic, Y ;

Esnault, C ;

Yang, T ;

Jacquet, M ;

Janin, J ;

Duguet, M ;

Perasso, R ;

Li, ZG .

NUCLEIC ACIDS RESEARCH, 2000, 28 (12) :2413-2419

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