LncRNA PTCSC3 Inhibits Tumor Growth and Cancer Cell Stemness in Gastric Cancer by Interacting with lncRNA Linc-pint

被引：31
作者：
Hong, Lei [1 ]
Wang, Haijuan [2 ]
Wang, Junyan [1 ]
Wei, Suju [1 ]
Zhang, Fan [1 ]
Han, Jing [1 ]
Liu, Yan [1 ]
Ma, Minting [1 ]
Liu, Chengyuan [1 ]
Xu, Yu [1 ]
Jiang, Da [1 ]
机构：
[1] Hebei Med Univ, Hosp 4, Dept Med Oncol, Shijiazhuang 050011, Hebei, Peoples R China
[2] Examinat & Training Ctr Hlth & Family Planning Co, Shijiazhuang 050051, Hebei, Peoples R China
来源：
CANCER MANAGEMENT AND RESEARCH | 2019年 / 11卷
关键词：
gastric cancer; lncRNA PTCSC3; lncRNA Linc-pint; growth; stemness; LONG NONCODING RNAS;
D O I：
10.2147/CMAR.S231369
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: The tumor suppressor role of lncRNA PTCSC3 has been reported in papillary thyroid carcinoma, our study aimed to investigate its involvement in gastric cancer. Methods: Tumor tissues and adjacent healthy tissues were collected from gastric cancer patients. Expression of PTCSC3 and lncRNA Linc-pint in these tissues was analyzed by RT-qPCR. The interaction between PTCSC3 and Linc-pint was analyzed by overexpression experiments. Cell proliferation and stemness were analyzed by CCK-8 assay and cell stemness assay, respectively. Results: PTCSC3 and lncRNA Linc-pint were both downregulated in tumor tissues than in adjacent healthy tissues of gastric cancer patients. Low levels of PTCSC3 and Linc-pint were closely correlated with poor survival. PTCSC3 and Linc-pint overexpression inhibited tumor growth and cancer cell stemness, while Linc-pint knockdown played an opposite role an attenuated the effects of PTCSC3 overexpression. Expression levels of PTCSC3 and Linc-pint were significantly correlated in tumor tissues but not in adjacent healthy tissues. Overexpression of PTCSC3 and Linc-pint upregulated the expression of each other. Conclusion: PTCSC3 inhibits tumor growth and cancer cell stemness in gastric cancer by interacting with lncRNA Linc-pint.
引用
收藏
页码：10393 / 10399
页数：7
相关论文
共 16 条
[1]   Non-coding RNA networks in cancer [J].
Anastasiadou, Eleni ;
Jacob, Leni S. ;
Slack, Frank J. .
NATURE REVIEWS CANCER, 2018, 18 (01) :5-18
[2]  
[Anonymous], 2015, GASTRIC CANC PRINCIP, DOI DOI 10.1007/978-3-319-15826-6_2
[3]   Gastric cancer [J].
Catalano, Vincenzo ;
Labianca, Roberto ;
Beretta, Giordano D. ;
Gatta, Gemma ;
De Braud, Filippo ;
Van Cutsem, Eric .
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, 2009, 71 (02) :127-164
[4]   Lymph nodes ratio based nomogram predicts survival of resectable gastric cancer regardless of the number of examined lymph nodes [J].
Chen, Shangxiang ;
Rao, Huamin ;
Liu, Jianjun ;
Geng, Qirong ;
Guo, Jing ;
Kong, Pengfei ;
Li, Shun ;
Liu, Xuechao ;
Sun, Xiaowei ;
Zhan, Youqing ;
Xu, Dazhi .
ONCOTARGET, 2017, 8 (28) :45585-45596
[5]   Long non-coding RNAs and complex diseases: from experimental results to computational models [J].
Chen, Xing ;
Yan, Chenggang Clarence ;
Zhang, Xu ;
You, Zhu-Hong .
BRIEFINGS IN BIOINFORMATICS, 2017, 18 (04) :558-576
[6]   Transcriptional and Post-transcriptional Gene Regulation by Long Non-coding RNA [J].
Dykes, Iain M. ;
Emanueli, Costanza .
GENOMICS PROTEOMICS & BIOINFORMATICS, 2017, 15 (03) :177-186
[7]   Long non-coding RNAs: new players in cell differentiation and development [J].
Fatica, Alessandro ;
Bozzoni, Irene .
NATURE REVIEWS GENETICS, 2014, 15 (01) :7-21
[8]  
Garitano-Trojaola Andoni, 2018, Oncotarget, V9, P12842, DOI 10.18632/oncotarget.24401
[9]   ImmunoScore Signature A Prognostic and Predictive Tool in Gastric Cancer [J].
Jiang, Yuming ;
Zhang, Qi ;
Hu, Yanfeng ;
Li, Tuanjie ;
Yu, Jiang ;
Zhao, Liying ;
Ye, Gengtai ;
Deng, Haijun ;
Mou, Tingyu ;
Cai, Shirong ;
Zhou, Zhiwei ;
Liu, Hao ;
Chen, Guihua ;
Li, Guoxin ;
Qi, Xiaolong .
ANNALS OF SURGERY, 2018, 267 (03) :504-513
[10]   Junk DNA and the long non-coding RNA twist in cancer genetics [J].
Ling, H. ;
Vincent, K. ;
Pichler, M. ;
Fodde, R. ;
Berindan-Neagoe, I. ;
Slack, F. J. ;
Calin, G. A. .
ONCOGENE, 2015, 34 (39) :5003-5011
12