Effect of miR-143-3p on C2C12 myoblast differentiation

被引：17

作者：

Du, Jingjing ^{[1
]}

Zhang, Yi ^{[2
]}

Shen, Linyuan ^{[1
]}

Luo, Jia ^{[1
]}

Lei, Huaigang ^{[3
]}

Zhang, Peiwen ^{[1
]}

Pu, Qiang ^{[1
,4
]}

Liu, Yihui ^{[1
]}

Shuai, Surong ^{[1
]}

Li, Qiang

Li, Xuewi ^{[1
]}

Zhang, Shunhua ^{[1
]}

Zhu, Li ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Anim Sci & Technol, Chengdu, Peoples R China

[2] Xichang Coll, Dept Anim Sci, Xichang, Peoples R China

[3] Univ Alberta, Dept Agr Food & Nutrit Sci, Edmonton, AB, Canada

[4] Sichuan Prov Gen Stn Anim Husb, Sichuan Prov Agr Dept, Chengdu, Peoples R China

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2016年 / 80卷 / 04期

关键词：

miR-143-3p; C2C12; myoblast differentiation; Wnt pathway; PARAXIAL MESODERM; CANCER CELLS; MUSCLE; PROLIFERATION; MICRORNAS; TRANSCRIPTS; EXPRESSION; MYOGENESIS; MUTATIONS; PROMOTES;

D O I：

10.1080/09168451.2015.1123604

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs are a class of 18-22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3 untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and -catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.

引用

页码：706 / 711

页数：6

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