A Clostridioides difficile bacteriophage genome encodes functional binary toxin-associated genes

被引：37

作者：

Riedel, Thomas ^{[1
,2
]}

Wittmann, Johannes ^{[1
]}

Bunk, Boyke ^{[1
,2
,3
]}

Schober, Isabel ^{[1
]}

Sproeer, Cathrin ^{[1
,2
,3
]}

Gronow, Sabine ^{[1
,2
,3
]}

Overmann, Joerg ^{[1
,2
,3
]}

机构：

[1] Leibniz Inst DSMZ, German Collect Microorganisms & Cell Cultures, Inhoffenstr 7B, Braunschweig, Germany

[2] North German Ctr Microbial Genom, Braunschweig, Germany

[3] German Ctr Infect Res DZIF, Partner Site Hannover Braunschweig, Braunschweig, Germany

来源：

JOURNAL OF BIOTECHNOLOGY | 2017年 / 250卷

关键词：

Firmicutes; Clostridium; Clostridium difficile; Clostridioides difficile; Bacteriophage; Binary toxin; Genome evolution; ADP-RIBOSYLTRANSFERASE; DIVERSITY; PLASMIDS; SEQUENCE; NEUROTOXIN; STRAIN; 630-DELTA-ERM; VISUALIZATION; EVOLUTION; ARTEMIS;

D O I：

10.1016/j.jbiotec.2017.02.017

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pathogenic clostridia typically produce toxins as virulence factors which cause severe diseases in both humans and animals. Whereas many clostridia like e.g., Clostridium perfringens, Clostridium botulinum or Clostridium tetani were shown to contain toxin-encoding plasmids, only toxin genes located on the chromosome were detected in Clostridioides difficile so far. In this study, we determined, annotated, and analyzed the complete genome of the bacteriophage phiSemix9P1 using single-molecule real-time sequencing technology (SMRT). To our knowledge, this represents the first C. difficile-associated bacteriophage genome that carries a complete functional binary toxin locus in its genome. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：23 / 28

页数：6

共 60 条

[1]

Abedon ST, 2005, EVOL BIOINFORM, V1, P97

[2] Virulence Plasmids of Spore-Forming Bacteria [J].