Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas

被引：143

作者：

Alvizo, Oscar ^{[1
]}

Nguyen, Luan J. ^{[2
]}

Savile, Christopher K. ^{[1
]}

Bresson, Jamie A. ^{[1
]}

Lakhapatri, Satish L. ^{[3
]}

Solis, Earl O. P. ^{[2
]}

Fox, Richard J. ^{[4
]}

Broering, James M. ^{[5
]}

Benoit, Michael R. ^{[1
]}

Zimmerman, Sabrina A. ^{[6
]}

Novick, Scott J. ^{[1
]}

Liang, Jack ^{[1
]}

Lalonde, James J. ^{[1
]}

机构：

[1] Codexis Inc, Redwood City, CA 94063 USA

[2] Calysta Energy Inc, Menlo Pk, CA 94025 USA

[3] Siluria Technol Inc, San Francisco, CA 94158 USA

[4] Pioneer HiBred Int Inc, Johnston, IA 50131 USA

[5] Novozymes Inc, Franklinton, NC 27525 USA

[6] BP Biofuels, San Diego, CA 92121 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 46期

关键词：

carbonic anhydrase; directed evolution; carbon capture; SATURATION MUTAGENESIS; ABSORPTION; STABILITY; KINETICS;

D O I：

10.1073/pnas.1411461111

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Carbonic anhydrase (CA) is one of nature's fastest enzymes and can dramatically improve the economics of carbon capture under demanding environments such as coal-fired power plants. The use of CA to accelerate carbon capture is limited by the enzyme's sensitivity to the harsh process conditions. Using directed evolution, the properties of a beta-class CA from Desulfovibrio vulgaris were dramatically enhanced. Iterative rounds of library design, library generation, and high-throughput screening identified highly stable CA variants that tolerate temperatures of up to 107 degrees C in the presence of 4.2 M alkaline amine solvent at pH > 10.0. This increase in thermostability and alkali tolerance translates to a 4,000,000-fold improvement over the natural enzyme. At pilot scale, the evolved catalyst enhanced the rate of CO2 absorption 25-fold compared with the noncatalyzed reaction.

引用

页码：16436 / 16441

页数：6

共 31 条

[1] Kinetics of the reactive absorption of carbon dioxide in high CO2-loaded, concentrated aqueous monoethanolamine solutions [J].