Pressure-Induced Dissolution and Reentrant Formation of Condensed, Liquid-Liquid Phase-Separated Elastomeric α-Elastin

被引：39

作者：

Cinar, Hasan ^{[1
]}

Cinar, Sueleyman ^{[1
]}

Chan, Hue Sun ^{[2
,3
]}

Winter, Roland ^{[1
]}

机构：

[1] TU Dortmund, Fac Chem & Chem Biol, Phys Chem Biophys Chem 1, Otto Hahn Str 40, D-44227 Dortmund, Germany

[2] Univ Toronto, Fac Med, Dept Biochem, Toronto, ON M5S 1A8, Canada

[3] Univ Toronto, Fac Med, Dept Mol Genet, Toronto, ON M5S 1A8, Canada

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2018年 / 24卷 / 33期

基金：

加拿大健康研究院;

关键词：

elastin; high-pressure chemistry; intrinsically disordered proteins; liquid-liquid phase separation; protein-protein interactions; HELIX-COIL TRANSITION; HYDROPHOBIC INTERACTIONS; PROTEIN; TEMPERATURE; POLYPEPTIDE; DYNAMICS; SPECTROSCOPY; BIOCHEMISTRY; AGGREGATION; STABILITY;

D O I：

10.1002/chem.201801643

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We investigated the combined effects of temperature and pressure on liquid-liquid phase separation (LLPS) phenomena of -elastin up to the multi-kbar regime. FT-IR spectroscopy, CD, UV/Vis absorption, phase-contrast light and fluorescence microscopy techniques were employed to reveal structural changes and mesoscopic phase states of the system. A novel pressure-induced reentrant LLPS was observed in the intermediate temperature range. A molecular-level picture, in particular on the role of hydrophobic interactions, hydration, and void volume in controlling LLPS phenomena is presented. The potential role of the LLPS phenomena in the development of early cellular compartmentalization is discussed, which might have started in the deep sea, where pressures up to the kbar level are encountered.

引用

页码：8286 / 8291

页数：6

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