SERS detection of cell surface and intracellular components of microorganisms using nano-aggregated Ag substrate

被引:50
作者
Lemma, Tibebe [1 ,2 ,3 ]
Saliniemi, Alex [1 ]
Hynninen, Ville [2 ,3 ]
Hytonen, Vesa P. [2 ,3 ]
Toppari, J. Jussi [1 ]
机构
[1] Univ Jyvaskyla, NanoSci Ctr, Dept Phys, POB 35, FI-40014 Jyvaskyla, Finland
[2] Univ Tampere, BioMediTech, Biokatu 6, FI-33520 Tampere, Finland
[3] Fimlab Labs, Biokatu 4, FI-33520 Tampere, Finland
基金
芬兰科学院;
关键词
Silver nano-aggregates; Bacteria; SERS; N-acetyl-D-glucosamine (NAG); N-acetylmuramic acid (NAM); ENHANCED RAMAN-SCATTERING; LOCUS VARIABLE-NUMBER; TANDEM-REPEATS ANALYSIS; TYPING METHOD; SILVER; SPECTROSCOPY; DISCRIMINATION; IDENTIFICATION; NANOPARTICLES; COLLOIDS;
D O I
10.1016/j.vibspec.2016.01.006
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The intracellular and cell surface composition and structural features of gram-positive and gram negative bacteria were identified using near-infrared surface-enhanced Raman scattering (SERS). The structural differences of components that reside in the cell envelope are manifested by their SERS spectra, e.g. gram-negative vs. gram-positive. Silver particles were used as a SERS substrate by exploiting the existence of strong local electromagnetic fields (hot spots) within nanoscale aggregates of the particles. The aggregation of silver nanoparticles was induced by magnesium ions. These hot spots reduce the screening length of the double layer. The obtained SERS spectra showed excellent quality having unprecedented high signal to noise ratio, which again enabled identification of numerous cellular components, such as proteins, polysaccharides, lipids and nucleic acids with excellent structure spectrum correlation. The SERS spectra associated with these features give rise to intense bands that provide information about the chemical composition of the bacterial cell envelope and can also detect low concentration compounds. We demonstrate the high sensitivity of our SERS based method for detection of bacteria and its capability to differentiate between bacterial species based on minor chemical differences on their outer cell envelope. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:36 / 45
页数:10
相关论文
共 31 条
[1]   ANOMALOUSLY INTENSE RAMAN-SPECTRA OF PYRIDINE AT A SILVER ELECTRODE [J].
ALBRECHT, MG ;
CREIGHTON, JA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (15) :5215-5217
[2]  
Austin L. A., 2015, NANO TODAY
[3]   Antibiotic-Resistant Bacteria: A Challenge for the Food Industry [J].
Capita, Rosa ;
Alonso-Calleja, Carlos .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2013, 53 (01) :11-48
[4]   Laser Tweezers Raman Spectroscopy Potential for Studies of Complex Dynamic Cellular Processes: Single Cell Bacterial Lysis [J].
Chen, De ;
Shelenkova, L. ;
Li, Y. ;
Kempf, C. R. ;
Sabeinikov, A. .
ANALYTICAL CHEMISTRY, 2009, 81 (09) :3227-3238
[5]   Optimized Multilocus Variable-Number Tandem-Repeat Analysis Assay and Its Complementarity with Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing for Listeria monocytogenes Clone Identification and Surveillance [J].
Chenal-Francisque, Viviane ;
Diancourt, Laure ;
Cantinelli, Thomas ;
Passet, Virginie ;
Tran-Hykes, Coralie ;
Bracq-Dieye, Helene ;
Leclercq, Alexandre ;
Pourcel, Christine ;
Lecuit, Marc ;
Brisse, Sylvain .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (06) :1868-1880
[6]   Surface enhanced Raman spectroscopy of bacteria coated by silver [J].
Efrima, S ;
Bronk, BV ;
Czégé, J .
ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY IV, PROCEEDINGS OF, 1999, 3602 :164-171
[7]   Silver colloids impregnating or coating bacteria [J].
Efrima, S ;
Bronk, BV .
JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (31) :5947-5950
[8]   Principal component analysis of bacteria using surface-enhanced Raman spectroscopy [J].
Guicheteau, Jason ;
Christesen, Steven D. .
CHEMICAL AND BIOLOGICAL SENSING VII, 2006, 6218
[9]   Surface-enhanced Raman scattering for the rapid discrimination of bacteria [J].
Jarvis, RM ;
Brooker, A ;
Goodacre, R .
FARADAY DISCUSSIONS, 2006, 132 :281-292
[10]   Discrimination of bacteria using surface-enhanced Raman spectroscopy [J].
Jarvis, RM ;
Goodacre, R .
ANALYTICAL CHEMISTRY, 2004, 76 (01) :40-47