Quantitative detection of Chlamydia psittaci and C-pecorum by high-sensitivity real-time PCR reveals high prevalence of vaginal infection in cattle

被引：74
作者：
DeGraves, FJ
Gao, DY
Hehnen, HR
Schlapp, T
Kaltenboeck, B
机构：
[1] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA
[2] Bayer AG, Anim Hlth, D-40789 Monheim, Germany
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 04期
关键词：
D O I：
10.1128/JCM.41.4.1726-1729.2003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.
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页码：1726 / 1729
页数：4
相关论文
共 12 条
[1]   Molecular diagnosis of Chlamydia pneumoniae infection [J].
Boman, J ;
Gaydos, CA ;
Quinn, TC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (12) :3791-3799
[2]   High-sensitivity quantitative PCR platform [J].
DeGraves, FJ ;
Gao, DY ;
Kaltenboeck, B .
BIOTECHNIQUES, 2003, 34 (01) :106-+
[3]   COMPARISON OF POLYMERASE CHAIN-REACTION, DIRECT IMMUNOFLUORESCENCE, CELL-CULTURE AND ENZYME-IMMUNOASSAY FOR THE DETECTION OF CHLAMYDIA-PSITTACI IN BULL SEMEN [J].
DOMEIKA, M ;
GANUSAUSKAS, A ;
BASSIRI, M ;
FROMAN, G ;
MARDH, PA .
VETERINARY MICROBIOLOGY, 1994, 42 (04) :273-280
[4]  
Galen RS, 1986, MANUAL CLIN LAB IMMU, V3rd, P966
[5]   Quantitative detection of Chlamydia spp. by fluorescent PCR in the LightCycler® [J].
Huang, J ;
DeGraves, FJ ;
Gao, D ;
Feng, P ;
Schlapp, T ;
Kaltenboeck, B .
BIOTECHNIQUES, 2001, 30 (01) :150-+
[6]  
Kaltenbock B, 1997, J CLIN MICROBIOL, V35, P1835
[7]   Use of synthetic antigens improves detection by enzyme-linked immunosorbent assay of antibodies against abortigenic Chlamydia psittaci in ruminants [J].
Kaltenboeck, B ;
Heard, D ;
DeGraves, FJ ;
Schmeer, N .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (09) :2293-2298
[8]  
PEREZMARTINEZ JA, 1986, AM J VET RES, V47, P1501
[9]  
Schachter J, 1999, CHLAMYDIA, P139
[10]   DOMINANCE OF CHLAMYDIA-PSITTACI-SPECIFIC IGG2 SUBCLASS IN THE HUMORAL IMMUNE-RESPONSES OF NATURALLY AND EXPERIMENTALLY INFECTED CATTLE [J].
SCHMEER, N ;
SCHNORR, KL ;
PEREZMARTINEZ, JA ;
STORZ, J .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1987, 15 (04) :311-322
12