Spectrofluorimetric determination of levofloxacin in tablets, human urine and serum

被引：83

作者：

González, JAO ^{[1
]}

Mochón, MC ^{[1
]}

de la Rosa, FJB ^{[1
]}

机构：

[1] Univ Seville, Fac Chem, Dept Analyt Chem, E-41012 Seville, Spain

来源：

TALANTA | 2000年 / 52卷 / 06期

关键词：

levofloxacin determination; micelle enhanced fluorescence; urine; serum; pharmaceutical tablets;

D O I：

10.1016/S0039-9140(00)00484-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A spectrofluorimetric method to determine levofloxacin is proposed and applied to determine the substance in tablets and spiked human urine and serum. The fluorimetric method allow the determination of 20-3000 ng ml(-1) of levofloxacin in aqueous solution containing acetic acid-sodium acetate buffer (pH 4) with lambda (exc) = 292 and lambda (em) = 494 nm, respectively. Micelle enhanced fluorescence improves the sensibility and allows levofloxacin direct measurement in spiked Human serum (5 mug ml(-1)) and urine (420 mug ml(-1)), in 8 mM sodium dodecyl sulphate solutions at pH 5. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：1149 / 1156

页数：8

共 13 条

[1]

[Anonymous], 1987, ANALYST, V112, P199

[2]

CHEN YD, 1999, WENXIU ZHONGGUO KANG, V23, P342

[3]

CHOHEN RK, 1987, QUINOLONE B, V3, P7

[4]

*CONS GEN COL OF F, 1998, CAT ESP FARM CD VERS

[5]

DAVIDSHON I, 1978, DIAGNOSTICO CLIN LAB

[6]

LOU S, 1998, YAOUXUE XUEBAO, V33, P937

[7]

Miller J. C., 1993, STAT ANAL CHEM

[8] AQUEOUS SOLUBILITIES OF SOME VARIOUSLY SUBSTITUTED QUINOLONE ANTIMICROBIALS [J].

ROSS, DL ;

RILEY, CM .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1990, 63 (03) :237-250

[9]

SI K, 1999, ZHONGGUO YAOXUEHUI, V34, P43

[10] Rapid stereospecific high-performance liquid chromatographic determination of levofloxacin in human plasma and urine [J].

Wong, FA ;

Juzwin, SJ ;

Flor, SC .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1997, 15 (06) :765-771

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