An investigation of the RWPE prostate derived family of cell lines using FTIR spectroscopy

被引：38

作者：

Baker, M. J. ^{[1
]}

Clarke, C. ^{[2
]}

Demoulin, D. ^{[1
]}

Nicholson, J. M. ^{[6
]}

Lyng, F. M. ^{[2
]}

Byrne, H. J. ^{[2
]}

Hart, C. A. ^{[3
]}

Brown, M. D. ^{[3
]}

Clarke, N. W. ^{[3
,4
,5
]}

Gardner, P. ^{[1
]}

机构：

[1] Univ Manchester, Manchester Interdisciplinary Bioctr, Ctr Instrumentat & Analyt Sci, Sch Chem Engn & Analyt Sci, Manchester M1 7DN, Lancs, England

[2] Dublin Inst Technol, FOCAS Res Inst, Dublin 8, Ireland

[3] Univ Manchester, Sch Canc Enabling Sci & Technol, Genito Urinary Canc Res Grp,Christie NHS Fdn Trus, Paterson Inst Canc Res,Manchester Acad Hlth Sci C, Manchester M20 4BX, Lancs, England

[4] Christie NHS Fdn Trust, Dept Urol, Manchester M20 4BX, Lancs, England

[5] Salford Royal NHS Fdn Trust, Dept Urol, Salford M6 8HD, Lancs, England

[6] STFC Daresbury Lab, Warrington WA4 4AD, Cheshire, England

来源：

ANALYST | 2010年 / 135卷 / 05期

基金：

英国工程与自然科学研究理事会;

关键词：

CANCER; MICROSPECTROSCOPY; GRADE;

D O I：

10.1039/b920385k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Interest in developing robust, quicker and easier diagnostic tests for cancer has lead to an increased use of Fourier transform infrared (FTIR) spectroscopy to meet that need. In this study we present the use of different experimental modes of infrared spectroscopy to investigate the RWPE human prostate epithelial cell line family which are derived from the same source but differ in their mode of transformation and their mode of invasive phenotype. Importantly, analysis of the infrared spectra obtained using different experimental modes of infrared spectroscopy produces similar results. The RWPE family of cell lines can be separated into groups based upon the method of cell transformation rather than the resulting invasiveness/aggressiveness of the cell line. The study also demonstrates the possibility of using a genetic algorithm as a possible standardised pre-processing step and raises the important question of the usefulness of cell lines to create a biochemical model of prostate cancer progression.

引用

页码：887 / 894

页数：8

共 30 条

[1]

[Anonymous], MATLAB CENTRAL FILE

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