N-ethylmaleimide-sensitive factor is associated with the nuclear envelope

被引：3

作者：

Mashima, J

Nagahama, M

Hatsuzawa, K

Tani, K

Horigome, T

Yamamoto, A

Tagaya, M ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Life Sci, Hachioji, Tokyo 1920392, Japan

[2] Niigata Univ, Fac Sci, Dept Biochem, Niigata 9502181, Japan

[3] Kansai Med Univ, Dept Physiol, Moriguchi, Osaka 5708506, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 274卷 / 02期

关键词：

N-ethylmaleimide-sensitive factor (NSF); soluble NSF attachment protein (SNAP); nuclear envelope; mitosis;

D O I：

10.1006/bbrc.2000.3162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Ethylmaleimide-sensitive factor (NSF) is an ATPase involved in many membrane fusion events within the exocytic and endocytotic pathways. In the present study we showed that NSF is associated with the nuclear envelope. Golgi-associated NSF was released from membranes upon incubation with Mg2+-ATP, reflecting the disassembly of a complex consisting of NSF, soluble NSF attachment proteins (SNAPs), and SNAP receptors (SNAREs), In contrast nuclear envelope-associated NSF in interphase cells was not released by the same treatment. During mitosis, however, it was released from nuclear membranes by Mg2+-ATP. These results suggest that the binding mode of nuclear membrane-associated NSF changes during the cell cycle, (C) 2000 Academic Press.

引用

页码：559 / 564

页数：6

共 25 条

[1] NUCLEAR MATRIX - ISOLATION AND CHARACTERIZATION OF A FRAMEWORK STRUCTURE FROM RAT-LIVER NUCLEI [J].